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A.A. Alrashidi Postgraduate Student, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: R.N. Kirakosyan Ph.D. (Biol.), RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail:

The research aim was to study the influence of a nutrient medium hormonal compositioof nutrient medium (2,4-D or NAA) and vitamin-mineral complex for receiving a suspension culture of Ashwaganda (Withania somnifera L.) in vitro. The suspension culture initiated from friable callus tissue type. It was established experimentally that to obtain a suspension culture quickly it is advisable to add nutrients vitamin-mineral complex on the background of auxin, either 2,4-D or NAA in a concentration of 2 mg/L. Under these conditions, well-formed proliferating, melkogravijnye suspension culture consisting of viable cells on average by 78%. The obtained suspension culture can be used to study the secondary metabolites and, in particular, withaferin A.

callus tissue
suspension culture
nutrient medium
growth regulators
in vitro

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  1. Hina T., Al, S., Asi M.R. Appraisal of an important Flavonoid, Quercetin, in callus cultures of Citrullus colocynthis // International Journal of Agriculture and Biology. 2012. № 14(4). S. 528−532.
  2. Alrashidi A.A., Solov'ev A.A., Kalashnikova E.A., Kirakosjan R.N. Vlijanie gormonal'nogo sostava pitatel'noj sredy na kallusogenez ashvagandy (Withania somnifera L.) in vitro // Voprosy biologicheskoj, meditsinskoj i farmatsevticheskoj himii. 2017. T. 20. № 9. S. 3−8.
  3. Shukla D.D., Bhattarai N., Pant B. In vitro Mass Propagation of Withania somnifera (L.) Dunal // Nepal Journal of Science and Technology. 2010. № 11. S. 101−106.
  4. Rani G., Virsk G.S., Nogpal A. Callus induction and plantlet regeneration in Withania somnifera (L.) Dunal. in vitro cell // Dev. Biol. Plant. 2003. № 39. S. 468−474.
  5. Suraj R.A., Bijaya P.A. Induction and Proliferation of in vitro Mass of Callus of Withania somnifera (L.) Dunal. // Research in Plant Sciences. 2013. V. 1. № 3. C. 58−61.
  6. Paudel S., Adhikari S. R., Pant B. Effect of colchicines on production of secondary metabolites from callus of Withania somnifera (L.) // Journal of Nepal Biotechnology. 2013. № 3(1). S. 15−18.
  7. Nosov A.M. Metody otsenki i harakteristiki rosta kul'tur kletok vysshih rastenij // V sb: Molekuljarno geneticheskie i biohimicheskie metody v sovremennoj biologii rastenij / Pod red. V.V. Kuznetsova, V.V. Kuznetsova, G.A. Romanova. M.: BINOM. 2011. S. 386–403.
  8. Handy M.T. Osobennosti obrazovanija steroidnyh glikozidov v kul'turah kletok Dioscorea deltoidea, Tribulusterrestris i Trigonella foenum-graecum: Avtoref. diss. … kand. biol. nauk. 2016. 23 s.