THE PREPARATION AND CHARACTERIZATION OF SUSPENSION CELL CULTURES OF WITHANIA SOMNIFERA L

DOI: https://doi.org/10.29296/25877313-2018-03-06
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Issue: 
3
Year: 
2018

A.A. Alrashidi Postgraduate Student, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: ahmad.aa.2013@mail.ru E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: kalash0407@mail.ru R.N. Kirakosyan Ph.D. (Biol.), RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: mia41291@mail.ru

The research aim was to study the influence of a nutrient medium hormonal compositioof nutrient medium (2,4-D or NAA) and vitamin-mineral complex for receiving a suspension culture of Ashwaganda (Withania somnifera L.) in vitro. The suspension culture initiated from friable callus tissue type. It was established experimentally that to obtain a suspension culture quickly it is advisable to add nutrients vitamin-mineral complex on the background of auxin, either 2,4-D or NAA in a concentration of 2 mg/L. Under these conditions, well-formed proliferating, melkogravijnye suspension culture consisting of viable cells on average by 78%. The obtained suspension culture can be used to study the secondary metabolites and, in particular, withaferin A.

Keywords: 
ashwaganda
callus tissue
suspension culture
nutrient medium
growth regulators
auxins
cytokinins
in vitro

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