THE INFLUENCE OF THE COMPOSITION OF THE NUTRIENT ENVIRONMENT ON THE GROWTH AND BIOSYNTHETIC INDICATORS OF THE SUSPENSION CULTURE OF RODYOLA ROSE (RHODIOLA ROSEA L.)

DOI: https://doi.org/10.29296/25877313-2018-08-02
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Issue: 
8
Year: 
2018

T.A. Savina Ph.D. (Biol.), Head of Department of Biotechnology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: savina-tatyana57@yandex.ru S.B. Myasnikova Research Scientist, Department of Biotechnology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: vilarnii@mail.ru P.S. Savin Ph.D.(Biol.), Learding Research Scientist, Department of Biotechnology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: savin-pavel@list.ru

In this study a special attention was paid to a possible exogenous regulation of the biological productivity of the cell culture of Rhodiola rosea components of the nutrient medium. As a result of the experiments it was found that high growth and biosynthetic in-dicators of a suspension culture of Rhodiola rosea can provide nutritional content in the substrate nitrogen and potassium in the ratio of 0.60:0.40 for the shares the amounts taken per unit and expressed in mg-atoms. On the basis of the conducted experiments the to-tal optimum of five elements with the composition of macrosoma was calculated: nitrogen: phosphorus : potassium: calcium : magne-sium, which helps to maintain the productivity of suspension culture at high level. The carbohydrates are the basis of all nutrient media for the cultivation of cell cultures of plants. The possibility of increasing the amount of phenylpropanoids by introducing in the culture medium of sucrose or glucose at a concentration equal to 4 %. It was estab-lished that the components of mineral-based carbohydrates sucrose and glucose equally support the growth and upregulate biosyn-thetic activity in the suspension culture of Rhodiola rosea

Keywords: 
suspension culture
mineral salts
carbohydrates
phenylpropanoid

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