IDENTIFICATION OF PHENOL COMPOUNDS IN BROCCOLI (BRASSICA OLERACEA L. VAR. ITALICA) BY UPLC/UV-MS/MS

Vegetables of Brassicaceae family, especially broccoli, are known to play an important role in human nutrition due to their phytochemicals, such as vitamins, minerals, glucosinolates, and phenolic compounds. In particular, it has been shown that broccoli potentially exert inhibitory activity against chronic diseases like cancer. Phenols such as flavonoids and hydroxycinnamic acid derivatives are widely distributed in plants and are important biologically active constituents. Risk of age-related diseases decreased in several epidemiologic studies, and the concept that flavonoids and other phenolic compounds are responsible is supported by some animal and in vitro studies. Flavonoids have powerful antioxidant activities, being able to scavenge a wide range of reactive oxygen, nitrogen, and chlorine species, such as superoxide, hydroxyl radical, peroxyl radicals. Glucosinolates are sulfur- and nitrogen-containing plant secondary metabolites. These have a common core structure containing a thioglucose group linked to a sulfonated aldoxime moiety and a variable side chain derived from amino acids. Sinapic acid is widespread in the plant kingdom and derivatives of sinapic acid are characteristic compounds in plants Brassica. Its shows antioxidant, antimicrobial, anti-inflammatory, anticancer, and anti-anxiety activity. This study identified phenolic compounds and glucosinalates from broccoli. These compounds were extracted from ethanolic extract of broccoli and characterized using ultra-performance liquid chromatography with photodiode array and triple-quadrupole mass spectrometry detector (UPLC-UV-MS/MS). Sinapic acid and several sinapoyl conjugates and compounds from class glucosinolate were identified based on retention time, UV spectra, MS fragmentation pattern. Compounds were identified in broccoli: sinapic acid derivatives: 1,2-disinapoylgentiobioside, 1-sinapoyl-2-feruloylgentiobioside, 1,2,2’-trisinapoylgentiobioside, 1,2’-disinapoyl-2-feruloylgentiobioside; glucosinolates: 4-methoxyglucobrassicin, neoglucobrassicin. This study showed that broccoli could be considered as good sources of food polyphenols. And could be conclusion about the prospects of broccoli as medicinal plant raw material was made.