LOCALIZATION OF PHENOLIC COMPOUNDS IN CELLS AND TISSUES OF MEDICINAL PLANTS (DIOSCOREA CAUCASIA LYPSKY, EUONYMUS NANA BIEB., ARISTOLOCHIA MANSHURIENSIS KOM.), CULTIVATED UNDER IN VITRO CONDITIONS

DOI: https://doi.org/10.29296/25877313-2019-05-09
Номер журнала: 
5
Год издания: 
2019

E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Department of Genetics, Breeding and Biotechnology, Moscow State Agricultural University MTAA named after K.A. Timiryazev E-mail: Kalash0407@mail.ru S.M. Zaytseva Ph.D. (Biol.), Associate Professor, Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin Smzaytseva@yandex.ru Doan Thu Thuy Ph.D. (Biol.), Associate Professor, Vietnam National University of Agriculture (Hanoi, Republic of Vietnam) E-mail: doanthuycgct@gmail.com R.N. Kirakosyan Ph.D. (Biol.), Associate Professor, Department of Genetics, Breeding and Biotechnology, Moscow State Agricultural University MTAA named after K.A. Timiryazev E-mail: Mia4129@mail.ru

The aim of the work is to study the localization of phenolic compounds in callus cultures of medicinal plants (Dioscorea caucasia Lypsky, Euony-mus nana Bieb., Aristolochia manshuriensis Kom), used as raw material sources in pharmacology. Methods and materials. The object of the study was callus cultures obtained from intact plants and regenerative plants (Dioscorea caucasia Lypsky., Euonymus nana Bieb and Aristolochia manshuriensis Kom.). Callus tissue was cultured on a nutrient medium containing Мurasiga and Skuga mineral salts and growth regulators at a temperature of 250 C and a 16-hour photoperiod, In alcohol extracts of callus cultures by spectrophotometric method, the content of the sum of soluble phenolic compounds (with Folin-Denis reagent), flavans (with vanillin reagent) and flavonols (with aluminum chloride) was determined. The localization of polyphenols was determined by histochemical methods (0.08% raster of Fast Blue reagent, reaction with vanillin reagent in hydrochloric acid vapor). Results. The resulting long-passable, well-proliferating callus cultures are characterized by a high ability to form polyphenols (flavans and fla-vanols). Flavanols were major components of the phenolic complex. In the process of culturing callus cultures, the level of accumulation of all studied classes of polyphenols is reduced in comparison with intact plant tissues. Summary. Under in vitro conditions, the species-specific ability to synthesize phenolic compounds is preserved, which is confirmed not only by quantitative determination of phenolic compounds, but also by histochemical studies. The polyphenols in cells of the calli were localized in micro- and macrovascular, in the intercellular spaces and cell walls. Among callus cells, epiblates with a central vacuole containing a large number of polyphenols in the form of an amorphous substance, as well as small and large-faceted inclusions, were occasionally encountered.

Ключевые слова: 
localization
phenolic compounds
flavanes
Dioscorea caucasia Lypsky
Euonymus nana Bieb.
Aristolochia manshuriensis Kom

Список литературы: 
  1. Alekseeva G.M., Belodubrovskaya G.A., Blinova K.F., Goncharov M.YU., Zhohova Е.V. Farmakognoziya. Lekarstvennoe syr’e rastitel’nogo i zhivotnogo proiskhozhdeniya / Pod red. G.P. Yakovleva Sankt-Peterburg. SpecLit. 2013.
  2. Kurkin V.A., Porojkov V.V. Fenilpropanoidy lekarstvennyh rastenij: prognoz antioksidantnoj i immunomoduliruyushchej aktivnosti // Sovremennye problemy nauki i obrazovaniya. 2015. № 2-2.
  3. Yurtaeva Е.A., Remezova I.P., Tyrkov A.G., Luzhnova S.A., Popova O.I. Suhoj ekstrakt list’ev lofanta anisovogo: poluchenie i analiz fenol’nyh soedinenij // Farmaciya. 2019. 68 (2): 28–32; https://doi.org/10.29296/25419218-2019-02-05.
  4. Zaprometov M.N. Fenol’nye soedineniya i ih rol’ v zhizni rasteniya. LVI Timiryazevskie chteniya. M.: Nauka. 1996. 45 s.
  5. Nosov A.M. Regulyaciya sinteza vtorichnyh soedinenij v kul’ture kletok rastenij. Biologiya kul’tiviruemyh kletok i biotekhnologiya rastenij / Pod redakciej R.G. Butenko. M. Nauka. 1991.
  6. Zaprometov M.N. Fenol’nye soedineniya i metody ih issledovaniya // Biohimicheskie metody v fiziologii rastenij. M.: Nauka. 1971. S. 185–197.
  7. Soukupova J., Cvikrova M., Albrechtova J. Histochemical and Biochemical Approaches to the Study of Phenolic Compounds and Peroxidases in Needles of Norway Spruce (Picea abies) // New Phytol. 2000. V. 146. P.403–414.
  8. Dubravina G.A., Zajceva S.M., Zagoskina N.V. Izmeneniya v obrazovanii i lokalizacii fenol’nyh soedinenij pri dedifferenciacii tkanej tissa yagodnogo i tissa kanadskogo v usloviyah in vitro // Fiziologiya rastenij. 2005.
  9. Tyukavkina N.A. Bioflavonoidy. M.: Izdatel’skij dom «Russkij vrach». 2002. 56 s.
  10. Zaprometov M. N. Nikolaeva T.N. Sposobnost’ izolirovannyh hloroplastov iz list’ev fasoli osushchestvlyat’ biosintez fenol’nyh soedinenij // Fiziologiya rastenij. 2003. T. 50. № 5. S. 699–702.
  11. Danielle Lugato, Mariela J. Simão, Renata Garcia, Elisabeth Mansur, Georgia Pacheco. Determination of antioxidant activity and phenolic content of extracts from in vivo plants and in vitro materials of Passiflora alata Curtis // Plant Cell, Tissue and Organ Culture. 2014. V. 118. Issue 2. P. 339–346.

It appears your Web browser is not configured to display PDF files. Download adobe Acrobat или click here to download the PDF file.