COMPARATIVE STUDY OF THE CONTENTS OF MAIN CLASSES OF PHENOLIC COMPOUNDS IN ROOTS AND RHIZOMES OF POTENTILLA ALBA, POTENTILLA RECTA AND POTENTILLA ANSERINA

DOI: https://doi.org/10.29296/25877313-2019-07-04
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Issue: 
7
Year: 
2019

N.A. Polyakov Ph.D. (Biol.), Senior Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: polakov@yandex.ru V.I. Ossipov Dr.Sc. (Biol.), Major Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow); Natural Chemistry Research Group, Department of Chemistry, FI-20014 University of Turku (Finland) E-mail: ossipov@utu.fi. V.A. Bykov Academician RAS, Major Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

The principal aim of the study was a comparative quantitation of the main classes of phenolic compounds (PC): gallo-and ellagitannins, procyanidins and prodelphinidines, p-coumaroyl and caffeoyl-derivatives of quinic acid, as well as kaempferol-, quercetin- and myricetin-glycosides, in the roots and rhizomes of Potentilla recta, Potentilla anserina and Potentilla alba. For analyzing plant PC was applied an ultra-efficient liquid chromatography combined with diode detector and high-resolution mass spectrometry. Content of various classes of PC was determined with application of multiple reaction monitoring (MRM) method. As a result, it was shown that the roots and rhizomes of the P. alba contain about 7,3% of procyanidins, but this species did not contain hydrolyzable tannins and other classes of PC. P. recta differs in approximately equal content of hydrolyzable and condensed tannins, but content of procyanidins was almost 2 folds less than that in P. alba. An even smaller amount of PC was found in the roots of P. anserina. At the same time, P. anserina was characterized by a significantly higher content of prodelfinidins, then procyanidins, which were typical for the other two species, P. alba and P. recta.

Keywords: 
Potentilla alba
Potentilla anserina
Potentilla recta
UPLC-DD-MSHR
phenolic compounds

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