DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR THE QUANTITATIVE ANALYSIS OF THE SUBSTANCE OF A NEW BIOLOGICALLY ACTIVE COMPOUND 3-[2-OXO-2-(4-PHENYL-1-PIPERAZINYL)ETHYL]-4(3H)-QUINAZOLINONE

DOI: https://doi.org/10.29296/25877313-2020-04-08
Issue: 
4
Year: 
2020

T.A. Gendugov Post-graduate Student, Department of Inorganic, Physical and Colloidal Chemistry, Pyatigorsk Medical and Pharmaceutical Institute - a branch of FGBOU VO VolgGMU of the Ministry of Health of Russia L.I. Shcherbakova Ph.D. (Pharm.), Associate Professor, Head of Department of Inorganic, Physical and Colloidal Chemistry, Pyatigorsk Medical and Pharmaceutical Institute - a branch of FGBOU VO VolgGMU of the Ministry of Health of Russia А.А. Ozerov Dr.Sc. (Chem.), Professor, Head of Department of Pharmaceutical and Toxicological Chemistry, Volgograd State Medical University A.A.Glushko Ph.D. (Pharm.), Senior Lecturer, Department of Inorganic, Physical and Colloidal Chemistry, Pyatigorsk Medical and Pharmaceutical Institute - a branch of FGBOU VO VolgGMU of the Ministry of Health of Russia

The purpose of the work is to study the possibility of using spectrophotometry in the UV region for the quantitative determination of a previously un-studied substance of 3- [2- (4-phenyl-1-piperazino) -2-oxoethyl] -quinazolin-4 (3H) -one (VMA-10 -21), as well as the development and validation of methods for its quantitative determination. Material and methods. In the course of the experiment, the analytical equipment was used: spectrophotometer SF-56, analytical balance RADWAG AS 220/C/2, measuring dishes of the 1st accuracy class. The optical density of the test solutions was measured at a wavelength of 275 nm in cu-vettes with a layer thickness of 10 mm. As a comparison solution used 1% solution of hydrochloric acid. Validation of the presented methodology was carried out according to indicators: linearity, precision and correctness. Processing of the experimental data was carried out according to OFS.1.1.0013.15 GF XIV edition. The specific absorption index was calculated using a standard sample of substance VMA-10-21, the purity of which was proved using the HPLC method. Results. The spectrum of the substance VMA-10-21 in the ultraviolet region was represented by two absorption bands with maxima at wavelengths of 235 and 275 nm. The use of a wavelength at a wavelength of 275 nm is justified. The specific absorption index at the selected wavelength was calcu-lated. A quantitative determination technique has been developed which, according to validation parameters, linearity, precision and correctness com-plies with the requirements of OFS.1.1.0012.15 GF of the XIV edition. Conclusions. The possibility of using spectrophotometry in the UV region for the quantitative determination of the substance VMA-10-21 was studied. A spectrophotometric method for the quantitative determination of the basic substance in the substance VMA-10-21 was developed and validated.

Keywords: 
spectrophotometry
quinazoline derivative
quantification
validation

References: 
  1. Il'nickij A., Proshchaev K. Vozrastno-situacionnaya depressiya. Vrach. 2018; 29(9): 48-49.
  2. Wittchen Н., Jacobi F., Rehm J. The size and burden of mental disorders and other disorders of the brain in Europe 2010 // Eur. Neuropsychopharmacol. 2011; 21(9):655679.
  3. Kodonidi I.P. Molekulyarnoe konstruirovanie i tselenapravlennyui sintezз N-zameshchennyukh proisvodnyukh 4-okso-1,4-digidropirimidina na osnove tormoznyukh neiromediatorov. Khimiko-farmatsevticheskii zhurnal. 2009; 43(10):3239.
  4. Patent № 2507199 (RF). Proizvodnyue khinazolina, obladayushchie antidepressivnoi i anksioliticheskoi aktivnostyu. Opubl. 20.02.2014. 12 s.
  5. Vlasov I.V. Zavodskaya laboratoriya. Diagnostika materialov. Spektrofotometricheskie metody v analize lekarstvennykh preparatov. 2011; 1:21.
  6. Gendugov T.A. Evraziiskii soyuz uchenykh. 2019; 11-2 (68):4647.
  7. Gosudarstvennaya Farmakopeya Rossiiskoi Federatsii. Izd. XIV. Т. 1. М. 2016. [Elektronnyi resurs]. Rezhim dostupa: http://feml.scsml.rssi.ru/feml
  8. Gavrilin M.V., Senchenko S.P. Validatsiya analiticheskilh metodik (metodicheskie ukaazaniya dlya aspirantov i studentov). GOU VPO Pyatigorskaya GFA Roszdrava. Pyatigorsk. 2008. 37 s.