STUDY OF CHEMICAL COMPOSITION OF VOLATILE SUBSTANCES OF PODOPHYLLUM PELTATUM CELLULAR CULTURE

DOI: https://doi.org/10.29296/25877313-2020-05-07
Issue: 
5
Year: 
2020

M.P. Kitaeva Post-graduate Student, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: kimape@mail.ru Ya.F. Kopytko Ph.D. (Pharm.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) T.A. Fedotcheva Ph.D. (Med.), All-Russian Scientific Research Institute of Medicinal and Aromatic Plants; Scientific Research Laboratory of molecular pharmacology, Russian National Research Medical University named after N.I. Pirogov (Moscow)

Podophyllum peltatum L. is used in modern medicine as a source of obtaining podophyllin. Podophyllin is a mixture of resinous substances, lignans such as aryl tetralin, the cytostatic compound of podophyllotoxin, which is the main active component of podophyllin, as well as flavonoids (quercetin and campferol). GC-MS is an optimal tool for studying the volatile compounds that make up the P. peltatum medicinal plant material. The purpose of the research was to study the chemical composition of podophyllin resin obtained from the cell culture of P. peltatum. The objective of the study is to conduct a chemical analysis of podophyllin resin by GC-MS. The object of the study was sus-pension cell cultures of Podophyllum peltatum L., obtained from the fetus, kidney, and root of the plant, which are in the collection of All-Russian Scien-tific Research Institute of Medicinal and Aromatic Plants. GC-MS analysis of the extracts was carried out on a Varian GC-220MS chromatograph-mass spectrometer with an ion trap mass analyzer. Quantification was carried out by the method of normalization by peak area. During methanol extraction of P. peltatum, the proportion of podophyllin in % of the dry weight of the cell culture was 15.98 ± 4.82% for the kidney cell line, 12.50 ± 3.32% for the fetus, and 18.38 ± 3.16% for the root. GC-MS analysis of the extract revealed a large number of volatile compounds (233–490): nitrogen-containing substances, alkaloids, esters of fatty acids, steroids, carbohydrates, glycosides, hydrocarbons, ketones, etc.

Keywords: 
Podophyllum peltatum
GС-MS
nitrogen compounds
steroids
carboxylic acids
carbohydrates
glycosides
ketones

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