EVALUATION OF ESSENTIAL METAL LEVELS IN SUBCUTANEOUS AND VISCERAL ADIPOSE TISSUE SAMPLES FROM PATIENTS WITH MORBID OBESITY: A PILOT STUDY

DOI: https://doi.org/10.29296/25877313-2021-12-08
Issue: 
12
Year: 
2021

A.V. Skalny Dr.Sc. (Med.), Professor, Sechenov University; RUDN University (Moscow, Russia) J.-S. Chang PhD, Taipei Medical University (Taipei, Taiwan) V.N. Nikolenko Dr.Sc. (Med.), Professor, Sechenov University (Moscow, Russia) S.-Y. Huang PhD, Taipei Medical University (Taipei, Taiwan) D.A. Zotkin Assistant, Sechenov University (Moscow, Russia) W. Wang PhD, Taipei Medical University (Taipei, Taiwan) A.A. Tinkov Ph.D. (Med.), Sechenov University; Associate Professor, RUDN University (Moscow, Russia) E-mail: tinkov.a.a@gmail.com

The objective of the study was to evaluate essential metal levels in subcutaneous and visceral adipose tissue samples from patients with morbid obesity. Adipose tissue samples were collected from 10 women with morbid obesity (BMI > 35) aged 30-50 y.o. Assessment of iron (Fe), copper (Cu), manganese (Mn), zinc (Zn), and selenium (Se) levels in adipose tissue samples was performed using inductively coupled plasma mass spectrometry. The obtained data demonstrate that iron and copper levels in visceral adipose tissue were 68% (р = 0,021) and 38% (р = 0,053) higher as compared to subcutaneous depot. No difference in tissue Mn, Se, and Zn levels were observed. The obtained values generally correspond to those reported in studies from Spain, Italy, and Lithuania. Correlation analysis demonstrated positive association between subcutaneous adipose tissue Fe, visceral adi-pose tissue Fe and Cu contents, and BMI values. Therefore, the obtained findings demonstrate heterogeneity of adipose tissue metal levels in patients with morbid obesity with predominant accumulation of Fe and Cu in visceral adipose tissue.

Keywords: 
adipocyte
heterogeneity
mass spectrometry

References: 
  1. Galic S., Oakhill J.S., Steinberg G.R. Adipose tissue as an endo-crine organ. Molecular and cellular endocrinology. 2010; 316(2): 129–139. https://doi.org/10.1016/j.mce.2009.08.018.
  2. Longo M., Zatterale F., Naderi J., Parrillo L., Formisano P., Raciti G. A., Beguinot F., Miele C. Adipose Tissue Dysfunction as De-terminant of Obesity-Associated Metabolic Complications. Interna-tional journal of molecular sciences. 2019; 20(9): 2358. https://doi.org/10.3390/ijms20092358.
  3. Tinkov A.A., Ajsuvakova O.P., Filippini T., Zhou J.C., Lei X.G., Gatiatulina E.R., Michalke B., Skalnaya M.G., Vinceti M., Aschner M., Skalny A.V. Selenium and Selenoproteins in Adipose Tissue Physiology and Obesity. Biomolecules. 2020; 10(4): 658. https://doi.org/10.3390/biom10040658.
  4. Fukunaka A., Fujitani Y. Role of Zinc Homeostasis in the Patho-genesis of Diabetes and Obesity. International journal of molecular sciences. 2018; 19(2): 476. https://doi.org/10.3390/ijms19020476.
  5. Tinkov A.A., Sinitskii A.I., Popova E.V., Nemereshina O.N., Gatia-tulina E.R., Skalnaya M.G., Skalny A.V., Nikonorov A.A. Alteration of local adipose tissue trace element homeostasis as a possible mechanism of obesity-related insulin resistance. Medical hypothe-ses. 2015; 85(3): 343–347. https://doi.org/10.1016/j.mehy.2015.06.005.
  6. González-Domínguez Á., Visiedo-García F.M., Domínguez-Riscart J., González-Domínguez R., Mateos R.M., Lechuga-Sancho A.M. Iron Metabolism in Obesity and Metabolic Syndrome. International journal of molecular sciences. 2020; 21(15): 5529. https://doi.org/10.3390/ijms21155529.
  7. Yang H., Liu C.N., Wolf R.M., Ralle M., Dev S., Pierson H., Askin F., Steel K.E., Magnuson T.H., Schweitzer M.A., Wong G.W., Lutsenko S. Obesity is associated with copper elevation in serum and tissues. Metallomics. 2019; 11(8): 1363–1371. https://doi.org/10.1039/c9mt00148d.
  8. Rodríguez-Pérez C., Vrhovnik P., González-Alzaga B., Fernández M.F., Martin-Olmedo P., Olea N., Fiket Ž., Kniewald G., Arrebola J.P. Socio-demographic, lifestyle, and dietary determinants of es-sential and possibly-essential trace element levels in adipose tissue from an adult cohort. Environmental pollution. 2018; 236, 878–888. https://doi.org/10.1016/j.envpol.2017.09.093.
  9. Malandrino P., Russo M., Ronchi A., Moretti F., Gianì F., Vigneri P., Masucci R., Pellegriti G., Belfiore A., Vigneri R. Concentration of Metals and Trace Elements in the Normal Human and Rat Thy-roid: Comparison with Muscle and Adipose Tissue and Volcanic Versus Control Areas. Thyroid, 2020; 30(2): 290–299. https://doi.org/10.1089/thy.2019.0244.
  10. Rodríguez-Pérez C., Gómez-Peña C., Pérez-Carrascosa F.M., Vrhovnik P., Echeverría R., Salcedo-Bellido I., Mustieles V., Željka F., Arrebola J.P. Trace elements concentration in adipose tissue and the risk of incident type 2 diabetes in a prospective adult cohort. Environmental pollution, 2021; 286: 117496. https://doi.org/10.1016/j.envpol.2021.117496.
  11. Kizalaite A., Brimiene V., Brimas G., Kiuberis J., Tautkus S., Zarkov A., Kareiva A. Determination of Trace Ele-
  12. ments in Adipose Tissue of Obese People by Microwave-Assisted Digestion and Inductively Coupled Plasma Optical Emission Spec-trometry. Biological trace element research. 2019; 189(1): 10–17. https://doi.org/10.1007/s12011-018-1450-7.
  13. Luong Q., Huang J., Lee K.Y. Deciphering White Adipose Tissue Heterogeneity. Biology. 2019; 8(2): 23. https://doi.org/10.3390/biology8020023.
  14. Ledoux S., Queguiner I., Msika S., Calderari S., Rufat P., Gasc J.M., Corvol P., Larger E. Angiogenesis associated with visceral and subcutaneous adipose tissue in severe human obesity. Diabetes. 2008; 57(12): 3247–3257. https://doi.org/10.2337/db07-1812.
  15. Chait A., den Hartigh L.J. Adipose Tissue Distribution, Inflamma-tion and Its Metabolic Consequences, Including Diabetes and Car-diovascular Disease. Frontiers in cardiovascular medicine. 2020; 7: 22. https://doi.org/10.3389/fcvm.2020.00022.
  16. Skalnaya M.G., Skalny A.V. Essential trace elements in human health: a physician’s view. Tomsk: Publishing house of Tomsk State University. 2018; 224 p.
  17. Nikonorov A.A., Skalnaya M.G., Tinkov A.A., Skalny A.V. Mutual interaction between iron homeostasis and obesity pathogenesis. Journal of trace elements in medicine and biology, 2015; 30: 207–214. https://doi.org/10.1016/j.jtemb.2014.05.005.
  18. Olechnowicz J., Tinkov A., Skalny A., Suliburska J. Zinc status is associated with inflammation, oxidative stress, lipid, and glucose metabolism. The journal of physiological sciences. 2018; 68(1): 19–31. https://doi.org/10.1007/s12576-017-0571-7.