IN VIVO COMPARISON OF THE BIOLOGICAL ACTIVITY OF TOPICAL METHYLPREDNISOLONE ACEPONATE FORMULATIONS USING VASOCONSTRICTION ASSAY

DOI: https://doi.org/10.29296/25877313-2022-05-08
Issue: 
5
Year: 
2022

M.S. Nesterov
Head of Laboratory for Bioanalytical Research,
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
(Moscow region, Krasnogorsk district, pos. Bright mountains, Russia)
E-mail: mdulya@gmail.com
D.V. Khvostov
Junior Research Scientist, Laboratory of Molecular Biology and Bioinformatics,
V.M. Gorbatov Federal Research Center for Food Systems, Russian Academy of Sciences (Moscow, Russia)
E-mail: daniil_hvostov@mail.ru
R.A. Ageldinov
Research Scientist, Laboratory for Bioanalytical Research,
Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia
(Moscow region, Krasnogorsk district, pos. Bright mountains, Russia)
E-mail: ageldinov@gmail.com

Methylprednisolone aceponate (MPA) is potent (class III) topical corticosteroid widely used for the treatment of inflammatory dermatoses. It’s peculiari-ties are exceptionally favorable safety profile, high anti-inflammatory activity and properties of prodrug – after application to the skin MPA is trans-formed into active metabolite methylprednisolone 17-propionate, and this process goes more intensively in the zone of inflammation thus limiting ster-oid effect on intact skin. After the patent expiration generic MPA drugs which didn’t pass through the full cycle of clinical trials have entered some markets, including Russian. Metabolic profile of these generics and it’s equivalence to the original product is unknown. Objective. To assess biological activity of various drug formulations of original (Advantan®) and generic (Komfoderm®) drugs of MPA using vasocon-striction assay in the in vivo model. Methods. Recommended doses of studied formulations were applied to the skin of laboratory animals (mini-pigs), and then experimental measure-ments of chromometric data were performed at specific timepoints (0.5 h, 3h, 6 h, 12 h, 24 h) using chromometer FRU WR-10 (8 mm). Results. Difference in the profile of biologic activity based on vasoconstriction assay data was noticed for all tested MPA drugs, ranging in descending order for lightness parameter L: Advantan® cream – Advantan® milk – Advantan® fatty ointment – Advantan® ointment – Komfoderm® К cream – Komfoderm® ointment – Komfoderm ® М2 ointment. Conclusions. Applicability of colorimetric method for evaluation of intensity of biologic effect of glucocorticoids based on the level of lightness of skin surface confirmed. Differences for this parameter for studied drugs detected. Noticed tendency to higher activity of the original drug in comparison to the generic.

Keywords: 
methylprednisolone aceponate
glucocorticoid
referent drug
generic
vasoconstriction assay

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