FORECAST ELECTROPHORETIC BEHAVIOR OF PHENOLIC COMPOUNDS IN TERMS OF CAPILLARY ZONE ELECTROPHORESIS

Issue: 
7
Year: 
2015

S.P. Senchenko
Ph.D. (Pharm.), Associate Professor, Department of Pharmaceutical and Toxicological Chemistry,
Pyatigorsk Medico-Pharmaceutical Institute − Branch to Volgograd State Medical University (Pyatigorsk)
E-mail: asp_nauka@mail.ru

Capillary electrophoresis (CE) is an updated and dynamically developing method of the analysis of the compound mixture of organic and inorganic substances including the objects of pharmaceutical industry. Despite the fact that practically all drugs can be subjected to analysis by resorting to different diversities of CE, nevertheless, the regularity of manifestations of drugs in electrolyte in accordance with their structure along with the lack of general approaches to developing methods of their analysis with CE realization are insufficiently studied. When developing the methods of the analysis of drugs which are structurally similar, it is of utmost significance to point out the possibility of preliminary modeling of the electrophoretic functioning, the theoretical calculation of electrophoretic mobility, the detection of the order of migration and the prognosis of dividing the analyzed components under different conditions. Therefore the aim of the research is to study the possibility of the prognosis of their electrophoretic functioning, taking into account the constants of ionization and molecular weight within the framework of biologically active substances of a number of simply single-charged phenols and phenolic glycosides. Theoretical electrophoretic mobility was calculated according to Offord’s model AZ/M2/3, where Z is a charge; A is a constant (identified empirically); M is molecular weight. The charge (Z) is offered to calculate from the constant ionization like the function from pH via Genderson-Hasselbakh’s equation. The Comparison resulted in the use of carbonate, borate and glycocol electrolytes shows that the best approximation between theoretical and practical findings is evident in case carbonate buffer solution is applied. The given correlation can be written by the equations of linear regression with the approximation ratio of 0,9788 and 0,8892 for simple phenols and phenolic glycosides respectively, thus enabling to apply these equations for theoretical calculations of electrophoretic mobility of both classes of the compounds. Moreover, the calculation to a considerable extent reflects electrophoretic realization of the substances within the range of ph carbonate electrolyte; both the order of migration of substances and the type of their division under different pH buffer solution are coincided. This gives the opportunity of predicting the main electrolyte pH with the aim of selecting the adequate conditions of the analysis under CZE.

Keywords: 
Keywords: capillary zone electrophoresis
Offord's model
phenols
phenolic glycosides
electrophoretic mobility.