COMPARATIVE ANALYSIS OF ESSENTIAL OILS SYNTHESIZED IN PETALS OF ROSA L. (ROSACEAE) AND A MYCELIUM OF EREMOTHECIUM KURTZMAN (EREMOTHECIACEAE)

Issue: 
7
Year: 
2015

A.I. Shpichka
Ph.D. (Biol.), Associate Professor, Penza State University
E-mail: ana-shpichka@yandex.ru
E.F. Semenova
Ph.D. (Biol.), Penza State University
E-mail: sef1957@mail.ru
E.V. Presnyakova
Ph.D. (Biol.), Associate Professor, Penza State University
E-mail: spl7@mail.ru

The interest to new sources for essential oils production, viz raw materials on a base of microalgae and microorganisms, is constantly growing [1, 2]. It was shown in our earlier works, micromycetes of Eremothecium genus are able to synthesize essential oil with a rose fragrance [3-8]. Therefore, the aim of the study is to analyze the essential oils produced by these organisms and processes of oilaccumulation in a comparative aspect. ВОПРОСЫ БИОЛОГИЧЕСКОЙ, МЕДИЦИНСКОЙ И ФАРМАЦЕВТИЧЕСКОЙ ХИМИИ, №7, 2015 43 Краткие сообщения Rosa gallica L., R. damascena L., 9 strains Eremothecium ashbyi Guilliermond and 5 strains E. gossypii Kurtzman were investigated. In the analysis of physicochemical properties and component compositions of the essential oils of Rosa sp. and Eremothecium sp. some characteristics are shown to be similar. It was marked that the essential oil synthesized by the Eremothecium species is closer to the oil extracted from petals of R. damascena L. and regulated by the international standard ISO 9842:2003 Rose Oil. The rose secretory structures are presented as oval endogenous cavities, located inside a parenchymal tissue, and exogenous grandular cells (accumulation of small essential oil drops) under a cuticle of an epidermis. In the study of the micromycetes the features of formation and development rates of some cell structures are revealed in hyphae of a mycelium. It was shown, there were electron clear striped lipid bodies in the hyphae of a daily submerged culture, and their quantity changed synchronously in accordance with the level of accumulation of essential oil components in a cultural liquid. In the 36…48 h cultivation period, the significant vacuolization was observed which was connected to monoterpene accumulation like in the oil-bearing rose [9, 10]. The presence of the lipid bodies of other type during all growth phases should be noted. They were dense (osmiophil) and had an oval shape. Their morphological features (quantity, sizes, location) characterized the mycelium functional activity as the increase of total osmiophylic ability of a protoplasm was established to be connected to the growth of total biosynthetical activity for every strain like in a case with the rose [9, 10]. Thus, it was revealed, the physicochemical features and the component composition of the essential oil produced by the Eremothecium species are close to ones of the oil extracted from petals of Rosa damascena L. The processes of oil-accumulation of the studied organisms are shown to be similar, and the oversynthesis of fragrant compounds is closely related to the cell functional activity which becomes evident because of the increase of the lipid drops quantity and the filling vacuoles by an osmiophil substance.

Keywords: 
Keywords: essential oil
Rosa
Eremothecium
oil-accumulation