COMPARATIVE KINETIC ANALYSIS OF ANTIRADICAL ACTIVITY OF APIGENIN AND NARINGENIN IN THE INTERACTION WITH THE RADICAL-CATION ABTS•+

Issue: 
1
Year: 
2016

I.R. Ilyasov
Ph.D. (Pharm.), Associate Professor at the Department of Organic chemistry, Faculty of Pharmacy,
I.M. Sechenov First MSMU (Moscow)
E-mail: igor@ilyasov.net
D.Yu. Demin
Рost-graduate Student, Laboratory of Heterocyclic Compounds, N.D. Zelinskiy Institute of Organic Chemistry RAS (Moscow)
E-mail: demindd@mail.ru
V.L. Beloborodov
Dr.Sc. (Pharm.), Professor, Head of the Department of Organic Chemistry, Faculty of Pharmacy, I.M. Sechenov First MSMU (Moscow)
E-mail: vlbe@list.ru
V.K. Kolkhir
Dr.Sc. (Med.), Chief Research Scientist, Department of Experimental and Clinical Pharmacology,
All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR) (Moscow)

The features of the antiradical behavior of naringenin and apigenin studied employing kinetic method assay based on their radical-cation ABTS•+ inhibition capacity. Kinetic curves of the accumulation of ABTS•+ in the presence of naringenin and apigenin showed the slow nature of their antiradical activity. At the initial stage of incubation apigenin shows higher antiradical activity compared to naringenin. Complete suppression of the ABTS•+ accumulation arises only to 8-12 min, not from the initial time of the incubation as for fast reacting antioxidants. Nevertheless, naringenin shows a more lasting and profound suppression of the accumulation of ABTS•+. To 300 minutes of incubation the level of radical cations is reduced by 84% compared to expected values in the absence of naringenin, and 73% for apigenin, when used at the same concentration.

Keywords: 
Key words: naringenin
apigenin
antiradical activity
induction period
ABTS.