ACTIVITY OF THE ENERGY SUPPLY MARKER ENZYMES OF BLOOD LEUCOCYTES’ MITOCHONDRIA UNDER THE CONDITIONS OF ALIMENTARY DEPRIVATION OF PROTEIN

Issue: 
1
Year: 
2016

O.N. Voloshchyuk
Ph.D. (Biol.), Assosiate Professor, Department of Biochemistry and Biotechnology,
Institute of Biology, Chemistry and Bioresources of Chernovtsi National University named by Yurii Fedkovych (Ukraine)
E-mail: oxbm@mail.ru
G.P. Kopuylchyuk
Dr.Sc. (Biol.), Professor, Head of Department of Biochemistry and Biotechnology,
Institute of Biology, Chemistry and Bioresources of Chernovtsi National University named by Yurii Fedkovych (Ukraine)
E-mail: kopilchuk@gmail.com
S.V. Bandura
Undergraduate, Department of Biochemistry and Biotechnology,
Institute of Biology, Chemistry and Bioresources of Chernovtsi National University named by Yurii Fedkovych (Ukraine)
E-mail: bandyrasveta@mail.ru

The activity of NADH: ubiquinone reductase [EC 1.6.5.3] and succinate dehydrogenase [EC 1.3.5.1] of blood leucocytes’ mitochondria under the conditions of alimentary deprivation of protein was studied in the work. The research was carried out on white non-linear rats divided depending on protein content in the ration into groups: 1 – rats, maintained on the low-protein diet; 2 – rats, maintained on the full-value semisynthetic ration. It is established, that long term lasting maintenance of rats under the conditions of alimentary protein deficiency is accompanied by the development of leucopenia on the background of disturbance of the functional activity of leucocytes’ mitochondria respiratory chain enzymatic markers. It is shown, that in leucocytes’ mitochondrial fraction of protein-deficiency rats the inhibition of NADH: ubiquinone reductase activity by 1.3 times is observed with the decrease of the succinate dehydrogenase activity by 2 times comparing to control group indices. Conclusion was made, that established consistent patterns of leucocytes’ mitochondria respiratory chain enzymes working are the fundamental for the supply of their functioning under the conditions of alimentary deprivation of protein and may be considered as one of the possible mechanisms of energy biotransformation system disturbances, determining the decrease of the immune system reactivity in case of protein deficiency.

Keywords: 
Key words: alimentary deprivation of protein
leucocytes
mitochondria
NADH: ubiquinone reductase
succinate dehydrogenase.