CHEMICAL COMPOSITION, APPLICATION AND STANDARDIZATION OF PETROSELINUM CRISPUM HOMEOPATHIC MOTHER TINCTURES

Issue: 
2
Year: 
2016

Ya.F. Kopytko
Ph.D. (Pharm.), Senior Research Scientist, Laboratory of Analytical Chemistry, Department of Standardization,
All-Russian Research Institute of Medicinal and Aromatic Plants (Moscow)
E-mail: yanina@kopytko.ru

The article presents data on the chemical composition, application in medicine, analysis of Petroselinum crispum. For quantitative estimation of phenols in the tincture homeopathic matrix Petroselinum crispum, prepared from fresh raw material (herba) maceration in 90% (by volume) with an alcohol, used the technique absorption spectrophotometry is carried out in the maximum at 313 nm using the specific absorption coefficient of apigenin in 70% alcohol equal to 980. Contents phenolic substances calculated as apigenin in the samples tincture Petroselinum crispum was from 0.051 to 0.148%. A study of volatile substances in the homeopathic tincture matrix Petroselinum crispum GLC-MS instrument on Varian 450GC-220MS (column FactorFOUR VF-5ms). The volatiles were removed with chloroform and then the solvent was evaporated and the residue dissolved in ethyl acetate. In tinctures detected 38 substances − ethyl cis-9, trans-11-octadecadienoic and linolenic acids (18.38 and 17.34% respectively), 3,7,11,15- tetramethyl-2-hexadecene-1-ol (12; 15%), myristicin (5.19%), cis-1-etiliden-octahydro-7a-methyl-1H-indene -1-etilidenoсtahydro-7a-methyl-1Hindene (4.80%) and apiol (3.25%). We have also found a series of terpene compounds cis-p-mentha-2,8-dien-1-ol, juniper camphor, β-phellandrene, izoaromadendren epoxide hydrocarbons hexadecene, heneicosane, docosane, eicosene, tricosene, tetracosane, phytol, falkarinol, free oleic acid esters of fatty acids, including cyclopropenoid fatty acids, isobergapten and isopimpenellin.

Keywords: 
Key words: Petroselinum crispum
homeopathic tincture
composition
quantification.