THE EXPERIMENTAL METHOD OF PRESERVING LIVER TRANSPLANT

Issue: 
2
Year: 
2016

A.S. Soharev
MD, surgeon of the Kuzbass Hepatology Center, Hospital № 3 behalf Podgorbunsky (Kemerovo)
E-mail: sokharevas@mail.ru
K.A. Krasnov
Ph.D. (Med.), Director of the Kuzbass Hepatology Center, Hospital № 3 behalf Podgorbunsky (Kemerovo)
A.V. Budaev
Dr.Sc. (Med.), Professor, Department Рathological Physiology, Kemerovo State Medical Academy
E.Y. Plotnikova
Dr.Sc. (Med.), Professor, Department of Education Primary Care Doctors, Course Director of Clinical Gastroenterology, Kemerovo State
Medical Academy

Liver transplantation is a standard treatment for patients with end-stage liver disease and unresectable liver tumors. The number of patients waiting for a liver transplant is increasing. This situation led to the adoption of extended criteria donor liver. The use of transplants from "marginal" donors has led to increased biliary strictures, acute and hyperacute rejection of organs and primary non-functioning graft, and thus the growth of retransplantation. Ischemic reperfusion injury is a major cause of graft initially nonfunctioning graft. Drug therapy is a promising technique for the prevention of disturbance in organ transplantation. This article studied and evaluated the use of the combined method for the prevention of disturbance in liver transplantation in laboratory animals. Studies carried out on rabbits divided into 3 groups of 20 animals. In the first group performed a liver preservation solution Custodiol. In the second group used a combined method: Custodiol and not oxygenated emulsion perfluororganic compounds in a ratio of 4:1. In the third group of liver preservation carried not oxygenated emulsion perfluororganic compounds. Preserved liver carried without perfusion method for 8 hours at a temperature of 4-5 °C. When comparing the second group after the graft preservation rabbit liver as compared with the first and third groups are statistically significantly lower cytolysis syndrome and anticoagulation. The most severe manifestations of the syndrome of cytolysis and heavy hypocoagulation observed in the third group. The use of the new combined method of preserving liver transplant can reduce ischemia-reperfusion injury of the liver, reduce the risk of primary nonfunctioning graft prevention hepatocellular failure in post-transplant period.

Keywords: 
Key words: liver transplantation
not oxygenated perfluororganic emulsion.