STUDY SOLUBILIZE PLANT EXTRACTS MIXTURES OF SORBITANS WITH DIFFERENT STRUCTURES

The aim of this work was to study solubilization of water-insoluble plant extracts (eucalimin and estifan) in mixed micelles sorbitans (Tween 20 and Tween 80) to increase their biopharmaceutical availability. Using a mixture of Tween 20/Tween-80, in a weight ratio of 1:4 respectively, based on their chemical composition. The resulting mixture contains surfactant molecules: 30−35% wt with saturated and 70-65% wt, with unsaturated hydrocarbon radicals, which allows preparation of a surfactant with mixed micelles containing a large volume of hydrophobic core. As a result of experimental studies, conditions for preparing nanodispension systems for plant extracts eucakalimin and estifan were determined. The optimum concentration of surfactant was determined − Tween 20 and 80, allowing us to prepare stable dispersions. The size and zeta potential of the prepared dispersion with nanosized particles was determined. Determination of allowable range interval of surfactant concentration in the system, determination of the surface tension (σ) of aqueous solutions of surfactants and surfactant mixtures with BAS was carried out. The results show a tendency of increasing particle size in an aqueous phase with increased concentration of surfactants, and BAS. When the content of BAS is 0.3% coarse particles with size range of 100 nm appeared, which indicates the transition from the micellar system to a miniemulsion system and stability decreases with increased concentration of BAS. Hence, to preserve the stability of micellar system at a concentration of surfactant = 0.1% the concentration of biologically active substances should not exceed 0.2%. Thus, a methodology for preparing nanoscale dispersions containing phytoextracts from eucakalimin and estifan was developed. As a stabilizer for the dispersion system a mixture of Tween 20 / Tween 80 was used, in a weight ratio of 1:4 respectively. These dispersions are characterized by aggregate and sedimentation stability, and can serve as a basis for the preparing new medicines that contain biologically active substances of natural origin.