ISOLATION AND ANALISYS OF FRACTIONS OF LIPOPHILIC AND PHENOLIC COMPOUNDS CONTAINED IN CHAGA
Issue:
5
Year:
2016
The fungus Inonotus obliquus (Pers.) Pil has been used as a folk medicine in Russia and Eastern Europe for more than four centuries. Inonotus obliquus
usually occurs as a sclerotium called a «Chaga» on the trunks of Betula trees.
In this work, the development of the methods of rational using of Chaga`s raw material was carried out. The methods aim to isolation of lipophilic
and phenolic compounds from Chaga. To increase the yield of these compounds raw materials with a different particle size were used. Also the
ratio of raw material and extracting agent, extraction time and the moistening of raw material were varied. The extraction of the raw material was carried
out by boiling. Ethyl acetate was chosen for Chaga`s treatment, because it has high ability to isolate both lipophilic and phenolic compounds.
The optimal conditions of the extraction were selected. It was found that the extraction of raw material with a 2.0 mm particle size allows to obtain the
maximum yield of lipophilic compounds – 1.2%, with a high content of terpenoids– 46.5 %, steroids – 12.1%. The extraction of moistened raw material
with a particle size less 2.0 mm can give phenolic compounds with yield of 0.16 % (2.3% of simple phenols, phenol carbonic acids and 7.2% of flavonoids).
Keywords:
Key words: Inonotus obliquus (Pers.) Pil
chaga
ethyl acetate
lipophilic compounds
phenolic compounds.