STUDY OF BIOLOGICAL ACTIVITY COMMON COCKLEBUR HERBAL EXTRACTS AND DRY SEPARATE FRACTIONS USING SPECIFIC BIOTEST ENZYMATIC SYSTEMS IN VITRO

Issue: 
6
Year: 
2016

G.R. Bushueva
Ph.D. (Pharm.), Senior Research Scientist, Department of Phytochemistry,
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)
E-mail: gulnara.khab@mail.ru
L.B. Strelkova
Ph.D. (Med.),Senior Research Scientist, Department of Biomedical Problems,
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)
N.V. Kondakova
Dr.Sc. (Biol.), Chief Research Scientist, Department of Biomedical Problems,
All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

The study of anti-inflammatory activity of dry extracts and individual fractions (chloroform, ethylacetate, butanol, water) derived from grass common cocklebur in vitro using enzyme bioassay systems based on inducible NO-synthase (iNOS) and xanthine oxidase (KO). Samples for study were obtained by extraction of grass common cocklebur 24, 40% and 70% ethanol and subsequent separation into fractions of liquid extraction. In iNOS-test significantly showed high anti-inflammatory activity of dry extracts and individual fractions obtained from the dry extract (extragent 70% ethanol) of the herbs of common cocklebur, which changed in a row: chloroform > ethylacetate > butanol > water. According TO the test identified inactivating effect on the xanthine oxidase biologically active substances contained in ethylacetate fraction of dry extract extracted with 70% ethanol from grass cocklebur common.

Keywords: 
Key words: xanthium strumarium (common cocklebur)
anti-inflammatory activity
enzymatic bioassay systems
xanthine oxidase
NO-synthase.