STUDY OF THE CHEMICAL COMPOSITION OF THE AERIAL PARTS OF PLANTAGO MAXIMA BY GAS CHROMATOGRAPHY MASS SPECTROMETRY

Issue: 
6
Year: 
2016

O.N. Nemerishina
Ph.D. (Biol.), Orenburg State Medical University
E-mail: olga.nemeresh@rambler.ru
A.A. Nikonorov
Dr.Sc. (Med.), Professor, Head of the Department of Biochemistry, Orenburg State Medical University;
Senior Research Scientist, Orenburg State University
A.A. Tinkov
Ph.D. (Med.), Orenburg State Medical University; Senior Research Scientist, Orenburg State University
T.L. Malkova
Dr.Sc. (Pharm.), Professor, Head of the Department of Toxicological Chemistry, Perm State Pharmaceutical Academy

Last decades demonstrated an increased interest to the search of novel phytochemical drugs characterized by combination of high efficiency and low toxicity, that is especially important in treatment of chronic diseases or prolonged rehabilitation process. Plantago L. based compositions are widely used in traditional and folk medicine. Plantago maxima Juss. ex Jacq. that is not included into the State Pharmacopoeia of the Russian Federation is growing in Orenburg region and its preparations are used as anti-inflammatory and antibacterial agents. Earlier the authors have studied certain therapeutic properties of P. maxima leaves. The aim of the current study was analysis of biologically active substances content in leaves of Plantago maxima Juss. ex Jacq. collected in South Ural region. The analyzed substances were measured using standard spectrophotometric methods and gas chromatography mass spectrometry. The obtained results demonstrate that P. maxima leaves contain a complex of biologically active substances. In detail, the leaves contain 3,85±0,08 mg/100g of iridoids, 6,84±0,11 mg/100g of tannides, 3,25±0,04 mg/100g of flavonoids, 63,68±0,19 mg/100g of ascorbic acid, 5,01±0,18 mg/100 g of tocopherol, 23,07±0,25 mg/100g of carotenoids. The method of gas chromatography mass spectrometry allows to determine tocopherol, phytol, squalene, sitosterol, Methyl-7-methylcyclopenta [c] pyran-4-carboxylate, 3-[3-Bromophenyl]-7-chloro-3,4-dihydro-10-hydroxy-1,9(2H,10H)-acridinedione in the raw materials (leaves) of Plantago maxima Juss. ex Jacq.

Keywords: 
Key words: medicinal plants
plantain
Plantago maxima
biologically active substances
GCMS.