THE STUDY OF STORAGE CONDITIONS OF THE SAPROPHYTE STRAIN CLAVICEPS PURPUREA № 04868244-008-2010-07-T-VILAR
Issue:
7
Year:
2016
We examined different options to storing the saprophyte strain Claviceps purpurea #04868244-008-2010-07-T-VILAR. The results
of our study confirm the ability to store strain of the fungus on stubble agar medium T2 in test tubes at a temperature of 4 °С for
up to 2 months without significant changes in viability. However, longer storage leads to a reduction in the number of conidia in the titer
from 2,7х108 to 1.1х105 units per ml of suspension and part of the conidia lose their viability under these storage conditions of
seed material. We find that storing the strain in the freezer in 40% aqueous glycerin solution ensures the preservation of growth potential
and biosynthesis at an acceptable level within 12 calendar months.
Keywords:
Key words: ergot alkaloids
Claviceps purpurea (Fries) Tulasne
viability
storage
indole derivatives.