EFFECT OF CHEMICAL MUTAGENS ON MORPHOLOGICAL AND CYTOGENETIC CHANGES CALENDULA OFFICINALIS L.

Issue: 
8
Year: 
2016

F.M. Hazieva
Ph.D. (Biol.), Head of Agrobiologic and Selection Department,
All-Russian Research Institute of Medicinal and Aromatic Plants (Moscow)
E-mail: vilar.6@yandex.ru
N.Y. Svistunova
Ph.D. (Biol.), Leading Research Scientist, Agrobiologic and Selection Department,
All-Russian Research Institute of Medicinal and Aromatic Plants (Moscow)
E-mail: aloevera09@mail.ru
T.E. Samatadze
Ph.D. (Biol.), Leading Research Scientist, Agrobiologic and Selection Department
All-Russian Research Institute of Medicinal and Aromatic Plants;
Associate Professor, Peoples’ Friendship University of Russia (Moscow)
E-mail: tsamatadze@gmail.com

The treatment chemical mutagen (dimethyl sulfate and diethyl sulfate of pot marigold seed was investigation. The influence of alkylating agents on the germinating ability and plant productivity is depend of mutagen concentration. For concentration of dimethyl sulfate mutagen is 0.04 %, for concentration of diethyl sulfate is 0.025%. The treatment of seed dimethyl sulfate mutagen in concentration 0.04% is prefer concentration 0.08%. Such plant with dimethyl sulfate mutagen has a higher amount of inflorescence, doubling inflorescence and crop yeld. The percent of pollen mother cell with disadvantage in metaphase and anaphase is higher for plant with dimethyl sulfate mutagen treatment and consist from 5.4 till 9.3%. Thus, the efficient of use chemical mutagen was submit for create of new material Calendula officinalis L. The dependence of influence chemical mutagen type on genotype pot marigold was obtained.

Keywords: 
Key words: selection
experimental mutagenesis DMS (dimethyl sulfate)
DES (diethyl sulfate)
analysis of meiosis.