DETERMINATION OF POLYSACCHARIDES AND FREE SUGARS SUM BY UV-SPECTROFOTOMETRY IN TUSSILAGO FARFARA LEAF

Issue: 
9
Year: 
2016

А.V. Nikulin
Ph.D. (Chem.), Associate Professor, Head of the Laboratory of Physicochemical Method,
Shared Research and Education Center, Peoples’ Friendship University of Russia (Moscow)
E-mail: Hr251@yandex.ruH
G.S. Теreshenko
Ph.D. (Chem.), Shared Research and Education Center, Peoples’ Friendship University of Russia (Moscow)
E-mail: Hgamiltonian@mail.ruH
О.G. Potanina
Dr.Sc. (Pharm.), Professor, Director of the Research and Development Department of the Shared Research and Education Center,
Peoples’ Friendship University of Russia (Moscow)
E-mail: Hmicroly@mail.ru

The procedure of polysaccharides and free sugars sum determination with phenol's reagent (after the acid hydrolysis) by UVspectrofotometry in the water extraction from the medicinal plant raw materials (Tussilago farfara leaf) is offered; the main metrological characteristics are estimated. The developed procedure is validated by standard addition method. The procedure has satisfactory metrological characteristics. The relative measurement error doesn't exceed 5%.

Keywords: 
Key words: medicinal herbs
polysaccharides
free sugars
leaves Tussilago farfara L.
phenol reagent
UV-spectrofotometry.