ORNITHINE DECARBOXYLASE ACTIVITY OF HUMAN SALIVA IN NORMAL CONDITIONS AND IN CANCER

Issue: 
9
Year: 
2016

L.V. Belskaya
Ph.D. (Chem.), Science Director, Research Department, JSC «ChemService» (Omsk)
E-mail: ludab2005@mail.ru
V.K. Kosenok
Dr.Sc. (Med.), Professor, Head of the Department of Oncology with Radiotherapy, Omsk Medical Academy
G. Massard
Dr.Sc. (Med.), Professor, Head of the Department of Thoracic Surgery and Transplantation, University of Strasbourg (France)

The study group included 288 patients (64.9% female, 35.1% male), and 100 healthy individuals, selected as a control group. Groups were formed according to the rules for conducting clinical trials after obtaining informed consent. As inclusion criteria were considered: patients age 30-70 years, the absence of any treatment at the time of the study, including surgery, chemotherapy or radiation, no signs of an active infection (including purulent processes), carrying out dental health. On the subsequent histological verification in the study group identified the following types of cancer pathology: breast cancer (20.0%), cervical and uterine cancer (17.8%), lung cancer (15.8%), cancer of the gastrointestinal tract (23,2%), cancer of the genitourinary system (13.5%), thyroid carcinoma (9.7%). In 27.8% of the applicants diagnosed with benign breast tumors, lung and thyroid. In all the patients before treatment was carried saliva sampling at one ml. Ornithine decarboxylase activity and an amino acid composition of the samples was determined. Increased ornithine decarboxylase activity noted. It is most pronounced during the early stages of tumor and metastasis. Metabolic changes during the formation of polyamines in the saliva in the background shows the cancer. In particular, it showed a reduction in total protein content in saliva, reducing the level of amino nitrogen, the total drop in the essential amino acids, and amino acid imbalance progressing. It was found a statistically significant decrease in the content of arginine with a simultaneous increase in arginase activity in the saliva of patients with benign and malignant diseases. Apparently, during the flow of the pathological process of tumor arginase predominant pathway of L-arginine, which in turn determines an increase in ornithine decarboxylase activity. Promising areas of practical application of the results may be the early diagnosis of cancer, monitoring of recurrence, as well as evaluation of treatment response.

Keywords: 
Key words: saliva
polyamines
ornithine
ornithine decarboxylase
arginase
amino acids
arginine
oncology.