ANTIRADICAL ACTIVITY OF APIGENIN, NARINGENIN AND GLUTATHIONE BINARY COMPOSITIONS

The antiradical activity (ARA) of apigenin‒naringenin, apigenin-glutathione, and naringenin‒glutathione compositions was investigated employing kinetic assay based on the capability of the antioxidants to inhibit ABTS•+ radical cation. The naringenin‒apigenin composition showed greater antioxidant power in comparison with individual flavonoids. ARA of apigenin‒glutathione 1:10 and naringenin‒glutathione 1:10 demonstrates stepwise inhibition of the radical cation ABTS•+ accumulation: full inhibition of ABTS•+ in the initial period due to the action of glutathione, the subsequent phase of ABTS•+ accumulation, which is obviously caused by the glutathione full consumption. The last phase is changing by a period of suppression of the ABTS•+ accumulation with a gradual decrease in its concentration caused by naringenin, apigenin action and presumably their active intermediates.