ANTIRADICAL ACTIVITY OF APIGENIN, NARINGENIN AND GLUTATHIONE BINARY COMPOSITIONS

Issue: 
10
Year: 
2016

Ph.D. (Pharm.), Associate Professor, Department of Organic Chemistry, Faculty of Pharmacy,
I.M. Sechenov First Moscow State Medicinal University
E-mail: igor@ilyasov.net
D.Yu. Demin
Рost-graduate Student, Laboratory of Heterocyclic Compounds, N.D. Zelinskiy Institute of Organic Chemistry RAS (Moscow)
E-mail: demindd@mail.ru
V.L. Beloborodov
Dr.Sc. (Pharm.), Professor, Head of the Department of Organic Chemistry, Faculty of Pharmacy,
I.M. Sechenov First Moscow State Medicinal University
E-mail: vlbe@list.ru
V.K. Kolkhir
Dr.Sc. (Med.), Chief Research Scientist, Department of Experimental and Clinical Pharmacology,
All-Russian Research Institute of Medicinal and Aromatic Plants (Moscow)
E-mail: kolkhir@pharmvilar.ru

The antiradical activity (ARA) of apigenin‒naringenin, apigenin-glutathione, and naringenin‒glutathione compositions was investigated employing kinetic assay based on the capability of the antioxidants to inhibit ABTS•+ radical cation. The naringenin‒apigenin composition showed greater antioxidant power in comparison with individual flavonoids. ARA of apigenin‒glutathione 1:10 and naringenin‒glutathione 1:10 demonstrates stepwise inhibition of the radical cation ABTS•+ accumulation: full inhibition of ABTS•+ in the initial period due to the action of glutathione, the subsequent phase of ABTS•+ accumulation, which is obviously caused by the glutathione full consumption. The last phase is changing by a period of suppression of the ABTS•+ accumulation with a gradual decrease in its concentration caused by naringenin, apigenin action and presumably their active intermediates.

Keywords: 
Key words: naringenin
apigenin
glutathione
binary compositions
antiradical activity
ABTS.