THE STUDY OF THE BIOCHEMICAL PARAMETERS OF BLOOD DURING THE SUBCHRONIC INJECTION OF OZONIZED SALINE IN THE EXPERIMENT

Issue: 
11
Year: 
2016

A.G. Soloveva
Ph.D. (Biol), Senior Research Scientist,
Privolzhsky Federal Research Medical Centre of the Ministry of Health of the Russian Federation (Nizhny Novgorod)
E-mail: sannag5@mail.ru
A.A. Ulanova
Ph.D. (Biol), Scientific Secretary, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)
S.P. Peretyagin
Dr.Sc. (Med.), Professor, Head of Department of Experimental Medicine,
Privolzhsky Federal Research Medical Centre of the Ministry of Health of the Russian Federation (Nizhny Novgorod)

Today ozone therapy is the whole direction in modern medicine. The therapeutic action of ozone is due to its high redox potential. The assessment of free radical processes and activity of oxidoreductases and their substrates in the blood allow to monitor the effectiveness of ozone therapy. However, the use of ozone is limited by the choice of doses. The aim of the work was to spend the study of the processes of free radical oxidation and energy metabolism of blood under the influence of different ozone concentrations during prolonged use (30 days) in the experiment. The material and methods. The experiment was conducted on 40 white Wistar rats. The animals were divided into 4 groups: group 1 – control (intact healthy rats), 2, 3, 4 groups – animals who daily were injected intraperitoneally with 1 ml of ozonated physiological solution with different doses of ozone in it (0,6 mcg, 2,0 mcg and 8 mcg). The parameters of free radical oxidation, the activity of lactate dehydrogenase and superoxide dismutase, the concentration of malonic dialdehyde, lactate and glucose were determined in the blood. The results. There was an increase in the utilization of glucose, lactate, pyruvate, the activation of oxidative phosphorylation, an increase in the resistance of erythrocyte membranes in the blood under the influence of ozone. Changes of biochemical parameters of blood of rats under exposure of ozone indicated on the possibility of ozone toxic effects on the body with the growth of its concentration. The conclusion. A dose-dependent effect of ozone was revealed. It was shown, that the metabolic optimum ozone dose is 0,6 mcg.

Keywords: 
Key words: ozone
lactate
glucose
lactate dehydrogenase
lipid peroxidation.