THIN-LAYER CHROMATOGRAPHY IDENTIFICATION OF PHENOLIC CONSTITUENTS IN BISTORTA RHIZOMATA

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Issue: 
1
Year: 
2017

K.N. Efimova
Ph.D. (Pharm.), Assistant, Department of Pharmacognosy, Saint-Petersburg State Chemical-Pharmaceutical Academy
E-mail: ksenia.rasarenova@pharminnotech.com
E.V. Zhokhova
Ph.D. (Pharm.), Associate Professor, Department of Pharmacognosy, Saint-Petersburg State Chemical-Pharmaceutical Academy
E-mail: elena.zhohova@pharminnotech.com

Bistorta major is a medicinal plant included in the State Pharmacopoeia of the USSR in 7th, 8th, 9th, 10th, 11th editions. Monograph «Rhizomata Bistortae» from the 11th edition of the State Pharmacopoeia of the USSR is the current document which determines the quality of Bistorta rhizome. In connection with elaborating monographs of new edition of the State Pharmacopoeia of Russian Federation the aim of our investigation was to update the exiting pharmacopeial article for Bistorta major rhizome. According to the literature gallic acid, chlorogenic acid, catechin were isolated from the root part of Bistorta major. By taking into account the contribution of phenolic constituents to the pharmacological effects of the investigated raw material in this paper the authors developed procedures for the quantitative determination if the mentioned monomers of tannins. The thinlayer chromatography was used for identifying phenolic constituents (gallic acid, chlorogenic acid, catechin) in rhizome of Bistorta major. Chromatographic separation of phenolic acids was achieved on a Sorbfil TLC Plates (PTLC-P-V-UV) using ethyl acetate toluene formic acid water (30:10:5:2) system as the mobile phase and diazotized sulfanilic acid reagent as spot detection. After spraying with reagent the chromatogram of the test solution showed a brownish-yellow and violet-pink areas at the level of standard gallic acid and chlorogenic acid respectively. Chromatography applying Sorbfil TLC Plates as stationary, butanol acetic acid water solvent system as a mobile phase, vanillinhydrochloric acid as spot detection reagent allowed the identification of catechin. The chromatogram of the test solution showed a bright red area at the level of standard catechin after detection. These approaches gave a good reproducibility for three samples of the raw material. Desighned TLC-method was included in the section «The thin-layer chromatography» of the monograph «Bistorta rhizomata» of new State Pharmacopeia of Russian Federation.

Keywords: 
Key words: Bistorta major
rhizomes
TLC
phenolic constituents
gallic acid
chlorogenic acid
catechin.

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