APPLICATION OF GC MS IN THE STUDY OF BIOLOGICAL ACTIVE SUBSTANCES SALVIA STEPPOSA

Issue: 
2
Year: 
2017

O.N. Nemerishina Ph.D. (Biol.), Orenburg State Medical University, Orenburg E-mail: olga.nemerishina@rambler.ru A.A. Nikonorov Dr.Sc. (Med.), Professor, Head of the Department of Biochemistry of the Orenburg State Medical University; Senior Research Scientist, Orenburg State University A.A. Tinkov Ph.D. (Med.), Orenburg State Medical University; Senior Research Scientist, Orenburg State University T.L. Malkova Dr.Sc. (Pharm.), Professor, Head of the Department of Toxicological Chemistry, Perm State Pharmaceutical Academy

Salvia L. species from the Lamiaceae family are traditionally used in European folk medicine and contain a high spectrum of bio-logically active compounds possessing antibacterial, antifungal, antiviral, astringent, and secretolytic activity. The main active com-pounds of S. officinalis are essential oils and tannic compounds. At the same time, Salvia stepposa Schost. growing in the steppe zone of South Urals is widely used by local inhabitants of the Orenburg region, Bashkortostan, Kazakhstan, and Khakassia in various diseas-es. However, the existing data on biologically active substance content in S. stepposa growing on the territory of the Orenburg region are insufficient. Therefore, the primary objective of the present study was investigation of bioactive substance content in Salvia step-posa using gas-chromatography and mass-spectrometry (GC-MS). Overground part (leaves) of S. stepposa, collected at flowering in 2012-2015 was used in the present study. After grinding and preparation of ethanolic extract the investigation was performed using gas-chromatography with mass-selective detection at gas chromatograph Agilent 7890A and mass-spectrometer Agilent 5975C with non-polar column (HP-5ms). The obtained mass-spectra were compared with the librarу-deposited spectra, using NIST08 library. Ap-plication of GC-MS allowed to reveal that ethanolic extracts of S. stepposa contain a number of novel biologically active substances in-cluding fitol, sitosterol, tocopherol, lupenone, and 2-[3-Bromophtnyl]-7-chloro-3.4-dihydro-10-hydroxy-1.9(2H.10H)-acridinedione. Hypothetically, some of these compounds may mediate anti-inflammatory, plastic, hypolipidemic activities of S. stepposa. At the same time, no flavonoid compounds were detected using GC-MS. It is proposed that more suitable methods for assessment of polyphenols are planar chromatography and UV-spectrometry.

Keywords: 
medicinal plants
Salvia stepposa
gas chromatography-mass spectrometry
phytol
sitosterol
lupenon
tocopherol.