THE DETERMINATION OF CATECHINS AND LEUCOANTHOCYANINS IN ACONOGONON DIVARICATUM

Issue: 
3
Year: 
2017

E.V. Ivanova Assistant, Omsk State Medical University E-mail: daisy891@mail.ru E.A. Luksha Ph.D. (Pharm.), Associate Professor, Omsk State Medical University G.I. Kalinkina Dr.Sc. (Pharm.), Professor, Siberian State Medical University (Tomsk) I.S. Pogodin Ph.D. (Pharm.), Associate Professor, Omsk State Medical University I.N. Korneeva Ph.D. (Chem.), Associate Professor, Omsk State Medical University I.A. Savchenko Ph.D. (Pharm.), Senior Lecturer, Omsk State Medical University A.F. Stepanov Dr.Sc. (Agrical.), Professor, Omsk State Agrarian University

The catechins (flavan-3-ols) are sufficiently large groups of organic compounds plant. Catechins have the antioxidant, anti-inflammatory, antibacterial, antispasmodic and neuroprotective effects. The leucoanthocyanins (flavan-3,4-diol), are precursors catechin, a correlation between the content flavandiols and flavanols in plants it was determined. The leucoanthocyanins exhibit antitumor and radioprotective activity, possess hypotensive, capillary protec-tion, anti-atherosclerotic action, as well as contribute to the efficient absorption of vitamin C. Promising for introduction in medical practice as a source of catechins and leucoanthocyanins is Aconogonon divaricatum L. – a perennial plant of the family Polygonaceae, which grows in the eastern regions of Siberia and is cultivated in the Omsk region. The aim of this study is to measure the content of catechins and leucoanthocyanins in the overground and underground organs of Aconogonon divaricatum L. The objects of the analysis were the overground and underground parts of Aconogonon divaricatum L. The quantitative determination of the amount of the complex of flavanols (catechin and leucoanthocyanins) was performed spectrophotometrically by measuring the absorbance of the colored reaction product obtained by reacting an ethanolic extract from a plant with vanillin reagent, by reacting an ethanolic extract from plants with a solution of hydrochloric acid in n-butanol. The content of catechins is calculated by the difference of the content of the sum of the complex flavanols and leucoanthocyanins. The greatest amount of catechins and leucoanthocyanins (%) found in underground organs (9,85 ± 0,22; 3,88 ± 0,08), flowers (7,50 ± 0,26; 1,47 ± 0,05) and fruits (8,01 ± 0,35; 1,33 ± 0,04)

Keywords: 
Key words: Aconogonon divaricatum
catechins
leucoanthocyanins
spectrophotometry.