THE SEARCH OF NEW PRODUCERS OF PROTEASES FROM FGBNU VILAR MICROMYCETES BIOCOLLECTION

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Issue: 
5
Year: 
2017

Z.K. Nikitina Dr.Sc. (Biol), Professor, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: nikitinaz@yandex.ru, I.K. Gordonova Ph.D. (Biol.), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: igordonova777@yandex.ru, Nguen Suan Tung Research Intern, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

The obtaining various biologically active substances using of biotechnological approaches is one of perspective directions of the modern scientific research. In recent years the list of such substances, including the proteases with different substrate specificity was significantly expanded. It should be noted, the different collagenases with their ability to hydrolyze collagen are among these enzymes. These enzymes are promising for use in medicine, cosmetics, various industries and agriculture. Most of the collagenases are produced abroad. The reviewed work is devoted to the search for promising producers of these enzymes, which may further solve the problem of import substitution. All of the above determines the novelty and relevance of the submitted work. In the present study, the ability of 5 not previously studied types of deuteromycetes from the collection of FGBNU VILAR to hydrolyze different collagen substrates was investigated by a screening method during the surface cultivation. The native collagen and the modified protein, having in-creased resistance to proteolysis, as an additional factor in the evaluation of proteolytic activity of microorganisms were used. The possibility of the adaptation of all deuteromycetes proteolytic systems for the modified collagen using as the substrate was shown. A com-prehensive study of the diameters of the colonies, zones of lysis, growth rates and indices of lysis of 5 micromycetes from biocollection FGBNU VILAR using native and modified collagen showed the greatest proteolytic potential of culture P. roqueforti (42), A. mangini (30) and P. martensii (47). These cultures are promising for use as the producers of the proteases.

Keywords: 
Key words: proteases
collagenases
micromycetes.

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