TEZIOGRAPHIC STUDY OF RATS’ BLOOD SERUM AFTER INTRAPERITONEAL INJECTION OF DINITROSYL IRON COMPLEXES WITH GLUTATHIONE LIGANDS

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Issue: 
5
Year: 
2017

A.K. Martusevich Dr. Sc. (Biol.), Privolzhsky Federal Medical Research Centre (Nizhny Novgorod); Kirov State Medical University; Association of Russian Ozone Therapeutists

The aim of this study is estimation study of the effect of intra-peritoneal introduction of dinitrosyl iron complexes with glutathi-one ligands on initiated crystallogenesis blood serum of rats. Peculiarities of teziographic picture of blood serum of rats after intraperi-toneal introduction of an aqueous solution of dinitrosyl iron complexes with glutathione ligands (dose of compound – 0.15; 0.30; 0.45 and 0.60 mM) and an aqueous solution of glutathione (0.15 mM). The study of influence of dinitrosyl iron complexes on crystallogenic properties of blood serum of rats was performed according to the method of comparative teziography using a special system of crite-ria. The main indicators evaluated in point scale, served as teziographic index, crystallinity, facia destruction degree and clearity of fa-cia marginal zone. 0.9% sodium chloride solution was used as crystallizator. The results of teziographic evaluation of serum samples of animals after the course of injections of dinitrosyl iron complexes in various concentrations clearly indicate that the activating effect of the compound against crystallogenic properties of biofluids are most pronounced for 0.3 - and 0.45-millimolar aqueous solutions of substance. This tendency is reflected in the growth of tezigraphic index and crystallinity of the samples and is accompanied by a mod-erate increase of the facia destruction degree of degradation and slight narrowing of the marginal zone. Given the fact that crystallo-genic properties represent composition and physico-chemical parameters of biological liquids, we can assume the dose-dependent metabolic response of the organism to administration of the compound. Thus, the optimal effect on metabolic processes have a relatively low dose of an agent that stimulates the antioxidant capacity of blood serum with a corresponding decrease in the intensity of lipid peroxidation processes, optimization of energy metabolism and increases the activity of the enzyme systems of detoxification.

Keywords: 
Key words: dinitrosyl iron complexes
teziography
crystallization
blood serum
biocrystallomics.

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