EFFICIENCY OF WATER AND WATER-ALCOHOL EXTRACTION OF CALENDULA OFFICINALIS RAW BY USING VARIOUS KINDS OF PRESOWING PROCESSING

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Issue: 
6
Year: 
2017

S.N. Shysh Research Scientist, SSI Central Botanical Gardens, NAS of Belarus (Minsk) E-mail: svetlana.shysh@gmail.com, H.G. Shutava Ph.D. (Biol.), Leading Research Scientist, SSI Central Botanical Gardens, NAS of Belarus (Minsk) , Z.E. Mazets Ph.D. (Biol.), Belarusian State Pedagogical University named M. Tank (Minsk)

Calendula officinalis L. is widely used in medicine, since a large number of therapeutic properties of its inflorescences. Preferably the composition of hydroalcoholic extracts of marigold inflorescences describes in literature. As a rule the aqueous extracts are differ in their composition and practically haven’t been studied. The goal of this work was to compare the composition of the aqueous and aqueous-alcoholic extraction of raw materials of calendula and fatty acid composition of its seeds after different types of pre-treatment: electromagnetic radiation (EMR) and 5-aminolevulinic acid (ALA). The object of the study was C. officinalis «Terry in 2000». A low power level of EMR for pre-processing (10 mW) in two frequency bands: 1) 64.0 -66.0 GHz; 2) 53,57-78,33 GHz, with exposures of 20, 12 and 8 minutes, and ALA in micro- and nanoconcentrations have been used. The determination of total polyphenols and flavonoids has been performed on spectrophotometer Agilent 8453. The qualitative and quantitative definition of flavonoids has been conducted on a chromatograph Agilent 1200. The separating components of flavo-noids samples has been performed on a column ZORBAX SB-Aqua C18 (3,0×100 mm, 1.8 microns). The identification of compounds of the inflorescences aqueous extract and seed oils has been evaluated by NMR spectrometer AVANCE-500 (Bruker). It was found that in the alcohol and water-alcohol extracts of this variety the main active ingredients are flavonoids (nartsisin, tifaniozin and kalendofla-vozid). The monosaccharides to 50%, proline and asparagine are prevailed in aqueous extracts. It is found that ALA (10−6%) caused a significant increase in the content of flavonoids.

Keywords: 
Key words: inflorescences
marigold seeds
alcoholic extracts
aqueous extracts
biochemical composition
polyphenols
flavo-noids
carotenoids
amino acids
fatty acid composition
electromagnetic radiation
aminolevulinic acid.

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