THE DEVELOPMENT OF THE MEMBRANES FROM LECITHIN AS MODELS OF BIOLOGICAL MEMBRANES

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Issue: 
7
Year: 
2017

N.S. Kajsheva Dr.Sc. (Pharm.), Professor, Faculty of Pharmaceutical and Toxicological Chemistry, Pyatigorsk Medic-Pharmaceutical Institute − Branch of Volgograd State Medical University E-mail: caisheva2010@yandex.ru A.S. Kajshev Ph.D. (Pharm.), Leading Specialist Inter-Regional Rosalkogolregulirovaniya Office for North Caucasian Federal District E-mail: kaishev2010@yandex.ru V.A. Karpenko Ph.D. (Pharm.), Associate Professor, Department of Pharmacy, Faculty of Postgraduate Education, Faculty of Pharmaceutical and Toxicological Chemistry, Pyatigorsk Medic-Pharmaceutical Institute − Branch of Volgograd State Medical University A.B. Samorjadova Ph.D. (Pharm.), Associate Professor, Faculty of Pharmaceutical and Toxicological Chemistry, Pyatigorsk Medic-Pharmaceutical Institute − Branch of Volgograd State Medical University

As models of the biological membranes are proposed a dense paper filters of the brand «blue ribbon» of the different diameters (5−20 cm) with the corresponding working area (15−300 cm2) allowing the dialysis of different volumes of fluid, and the pore diame-ter (0,35−0,40 nm) corresponding to the diameters of pores of biological membranes. Experimentally, the optimum conditions for the processing of paper filters lecithin: lecithin solvent − boiling ethanol 95% (ratio of solubility of lecithin 6.3 g/100 ml ethanol 95%); flow rate at 6.3% alcohol solution of lecithin to impregnate 10 filters with a diameter of 5.0−20.0 cm − 80−130 ml; the duration of treatment of the filters with a solution of lecithin is 48 hours. Comparative evaluation of lecithin membranes held in matching cello-phane and a biological membrane (parietal sheet of peritoneum of the rat), for example, polysaccharides (pectin, sodium alginate). The criterion of evaluation was the degree of dialysis, including the degree of dialysis, taking into account the duration of the process. When applying the lecithin membrane and parietal sheet of peritoneum of the rat during dialysis as pectin and sodium alginate were obtained comparable results: the average values given degree of dialysis differ 2.7% and 4.0%, respectively. When using plastic films differences with the parietal sheet of peritoneum of the rat according to the average values given degree of dialysis of pectin reach 11.6%, sodium alginate 17.2% per cent, and the high performance is attributed to the inert nature of the membranes. Along with the possibility of replacing biological membranes, lecithin membranes are resistant to the processes of decay, decomposition, oxidation, variation of the area of the working surface and speed of dialysis, duration of storage and use, the possibility of manufacturing as re-quired, ease of manufacture, availability of materials and reagents.

Keywords: 
biological membranes
lecithin
a dialysis
polysaccharides.

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