IN VITRO CULTURE SILYBUM MARIANUM (L.) AS A POTENTIAL SOURCE OF TARGET BAS

Issue: 
7
Year: 
2017

O.V. Kovzunova Research Scientist, Central Botanical Gardens NAS of Belarus (Minsk) E-mail: olga-kopa@mail.ru N.V. Pushkina Research Scientist, Research Institute for Nuclear Problems of Belarusian State University (Minsk) S.G. Аzizbekian Senior Research Scientist, Institute of Physical Organic Chemistry NAS of Belarus (Minsk)

The development of effective ways to obtain the most raw materials by the method of culture of cells and tissues and the crea-tion of new technologies plant-based preparation is a topical scientific and practical problem whose solution will make an important contribution to the development of pharmaceutical technology and increase the output of essential health medicines was revealed. In this regard, the purpose of our study was to investigate changes of metabolism in in vitro cultures of S. marianum obtained from plants of the two races, the effects of modifiers of different nature, based on a content analysis of target biologically active substances on the basis of which a number of pharmacological preparation was developed. It was used for the analysis of callus and suspension culture 4th and 27th passage, cultured on MS (2 mg/l 6-Benzylaminopurine and 1 mg/l 1-naphthylacetic acid). Processing pre-sowing microwave treatment (a physical modifier PSMT) was carried out in the mil-limeter wavelength range with a capacity of 10 mW and treatment time of 20 minutes. The preparation of nanoparticles of metals «Nanoplant – Co, Mn, Cu, Fe» (chemical modifier) — a concentration of 0.01 mg/L. Extraction of flavonolignans was carried out by the standard method. The analysis of the total content of flavonolignans in callus and suspension culture of S. marianum showed that PSMT and com-plex preparation of nanoparticles of metals has a positive stimulating effect on the biosynthesis of target biologically active substances and flavolignans content increases from 19 to 172%, relative to control. Long cultivated suspension cultures are more responsive to PSMT, and callus culture are more responsive to preparation of nanoparticles of metals. Selected treatment mode and the concentra-tion of metal nanoparticles (0.01 mg/l) can be used to increase the biosynthesis of target compounds in in vitro cultures of S. maria-num.

Keywords: 
in vitro
suspension culture
callus cultures
flavolignany
biologically active substances.