THE EFFECT OF HORMONAL COMPOSITION OF NUTRIENT MEDIUM FOR CALLUSOGENESIS AND MORPHOGENESIS OF ASHWAGANDA (WITANIA SOMNIFERA) IN VITRO

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Issue: 
9
Year: 
2017

A.A. Alrashidi Post-graduate Student, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: ahmad.aa.2013@mail.ru, A.A. Soloviev Dr.Sc. (Biol.), Professor, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: a.soloviev70@gmail.com, E.A. Kalashnikova Dr.Sc. (Biol.), Professor, Department of Genetics, Biotechnology, Breeding and Seed Production, RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: kalash0407@mail.ru , R.N. Kirakosyan Ph.D. (Biol.), RSAU-MTAA named after K.A. Timiryazev (Moscow) E-mail: mia41291@mail.ru

The research aim was to study the influence of a nutrient medium hormonal composition on morphogenetic potential of As-wanaganda isolated tissues (Withania somnifera) in vitro. The research object was ashwaganda seeds and plants-regenerants. To ob-tain a sterile culture, seeds were sterilized with a solution of mercuric chloride at a concentration of 0.1% for 10 minutes, followed by their cultivation on the Murashige and Skoog (MS) hormone-free nutrient medium to get sterile shoots and plants. Callus tissue was obtained from leaf explants, isolated from in vitro plants. It was established experimentally, that to obtain quickly a well proliferating callus tissue, it is advisable to add 2.4-D to the medium at the concentration of 2 mg /l in combination with kinetin of 0.5 mg /l. Under these conditions a loose callus tissue is formed, which can later be used to produce suspension culture in order to obtain secondary metabolism substances. To get regenerating plants from callus tissue, it is necessary to apply BAP at a concentration of 0.5 mg /l.

Keywords: 
Key words: ashwaganda
callus tissue
nutrient medium
growth regulators
auxins
cytokinins
plants-regenerants
in vitro.

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