THE INFLUENCE OF THE PERINATAL ALCOHOL ON THE AMINO-ACID RANGE OF BLOOD SERUM OF PREGNANT WOMEN AND THEIR OFFSPRING

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Issue: 
10
Year: 
2017

A.V. Skalny Dr.Sc. (Med.), Professor, Leading Expert, P.G. Demidov Yaroslavl State University; Head of Department, RUDN University (Moscow); Director, Institute of Bioelementology, Orenburg State University E-mail: skalny3@microelements.ru Y.N. Lobanova Ph.D. (Biol.), Senior Lecturer, RUDN University (Moscow) E-mail: lobanova_ju@mail.ru A.R. Grabeklis Ph.D. (Biol.), Leading Expert, P.G. Demidov Yaroslavl State University E-mail: andrewgrabeklis@gmail.com E.V. Kiyaeva Ph.D. (Med.), Senior Research Scientist, Institute of Bioelementology, Orenburg State University E-mail: elena_sap@mail.ru A.A. Tinkov Ph.D. (Med.), Leading Expert, P.G. Demidov Yaroslavl State University E-mail: tinkov.a.a@gmail.com

The primary objective of this study was to assess the amino acid spectrum of blood serum of the pregnant women abusing alco-hol and their children. 30 pregnant women (the 3rd trimester, an age 24±3) were examined, including 15 suffered from alcohol abuse, and 30 children aged 1 year, who were exposed to perinatal ethanol influence and those who were not. Amino acid concentrations were determined by high performance liquid chromatography using a PerkinElmer S200 chromatograph (Perkin Elmer, USA) after car-rying out sample preparation using cartridges containing a special sorbent based on porous silica, surface modified with a layer of fluoroplastic. In women with alcohol abuse, there was a significant decrease in the concentration of arginine and glutamate by 25% and 33% relative to control, respectively. As in pregnant women, children who underwent perinatal exposure to alcohol had a signifi-cant 42% and 29% reduction in glutamate and serine serum concentrations. The presence of a close to significant decrease in the concentration of alanine, asparagine and hydroxyproline, due to perinatal exposure to alcohol by 17%, 36%, and 31% was reveled. A reliable direct correlation between maternal and children's indexes was noted in the case of arginine, glutamate and valine. The results of the study showed that the abuse of alcohol by pregnant women leads to a significant decrease in the concentration of arginine and glutamate in the serum, and the subsequent development of glutamate and serine deficiency in the blood of offspring. In this case, the concentration of arginine, glutamate and valine in the maternal and infant serum is characterized by a reliable direct correlation. Con-sidering a role of these amino acids in development of an organism in general and the central nervous system in particular, it is fair to assume that observed imbalances at least partially cause the development violations associated with the perinatal influence of alcohol.

Keywords: 
pregnancy
children
alcohol
amino-acid range
blood serum.

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