MARKERS OF ENERGY METABOLISM IN DESTABILIZATION OF CIRCADIAN RHYTHMS

Issue: 
11
Year: 
2017

E.G. Batotsyrenova
Ph.D. (Biol.), Senior Research Scientist, Laboratory Biochemical Toxicology and Pharmacology,
Institute of Toxicology Federal Medico-Biological Agency (St. Petersburg)
E-mail: bkaterina2009@yandex.ru V.A. Kashuro
Dr.Sc. (Med.), Head of Laboratory Biochemical Toxicology and Pharmacology,
Institute of Toxicology Federal Medico-Biological Agency (St. Petersburg)
E-mail: kashuro@yandex.r
M.B. Ivanov
Dr.Sc. (Med.), Director of Institute of Toxicology Federal Medico-Biological Agency (St. Petersburg)
E-mail: m.b.ivanov@toxicology.ru

As a result of undertaken study it was shown that under physical exercise in destabilization of circadian rhythms occur increasing the concentration of HIF1α in the serum of rats of experimental groups than those in the serum of rats in the control group. HIF1α is activated in the physiologically important points regulation of oxygen ways, providing a quick and adequate responses to hypoxic stress genes that includes the process of angiogenesis, vasomotor control, energy metabolism, erythropoiesis and apoptosis. This change of concentration involves the development of hypoxia in conditions of dark and light deprivation. During of intense physical activity in muscle under anaerobic conditions formed elevated quantity of lactate, which in the cytosol of hepatocytes turns into pyruvate. While formed molecules NADH++ H+. Next pyruvate is transported into the mitochondria, where in two reactions is formed phosphoenolpiruvate (PEP). The study noted a significant increase in serum phosphoenolpiruvate carboxykinase (EC 4.1.1.32) activity compared to similar indicators under normal lighting. PEPKK activity in conditions of desynchronosis suggests activation of gluconeogenesis enzymes synthesis with a view to ensuring the restoration of energy exchange alternatives. Analysis of data leads to a conclusion that cell clock function may be applied to enzymes responsible for cell substrates energy metabolism.

Keywords: 
physical exercise
energy metabolism
hypoxia
phosphoenolpyruvate carboxykinase
circadian rhythms.