LIPID PROFILE AND FREE RADICAL OXIDATION IN NON-ALCOHOLIC FATTY LIVER DISEASE

Issue: 
11
Year: 
2017

Assistant, Department of Biochemistry, Orenburg State Medical University;
aspirant, South Ural State Medical University (Chelyabinsk)
E-mail: gatiatulina@hotmail.com
E.V. Popova
Ph.D. (Med.), Associate Professor, Department of Biochemistry, Orenburg State Medical University; St Joseph University in Tanzania,
St. Joseph College of Health Sciences (Dar es Salaam, Tanzania)
V.S. Polykova
Dr.Sc. (Med.), Professor, Orenburg State Medical University E.F. Agletdinov
Dr.Sc. (Med.), Professor, Bashkir State Medical University (Ufa)
E.A. Nikonorova
Assistant, Department of Medical Elementology, RUDN University (Moscow)
A.A. Tinkov
Ph.D. (Med.), Assistant, Department of Biochemistry, Orenburg State Medical University;
Engineer-researcher, P.G. Demidov Yaroslavl State University
E-mail: tinkov.a.a@gmail.com
А.А. Nikonorov
Dr.Sc. (Med.), Head of Department of Biological Chemistry, Orenburg State Medical University

Diet-induced liver steatosis is associated with metabolic syndrome, insulin resistance and cardiometabolic disorders such as abnormal lipoproteins metabolism, excessive accumulation of lipids in hepatocites and activation of free radicals formation. Consequently, the aim of the present study was to assess lipid spectrum and oxidative stress markers in rats with diet-induced non-alcoholic fatty liver disease (NAFLD). The animals were divided into two equal groups and fed standard diet (10% calories from fat) or high-calorie diet (HCD) (60% calories from fat in chow and 10% sucrose solution) for 6 weeks. Serum of blood and liver samples used for estimation of lipid spectrum and some markers of protein modification and lipids peroxidation. The obtained data showed that histologically verified NAFLD led to atherogenic dyslipidemia in rats, absence of free radical oxidation in serum, and a significant decrease in total thiols concentration in liver. This fact may indicate the formation of local oxidative stress in liver of rats fed high-calorie diet.

Keywords: 
liver; non-alcoholic fatty liver disease; high-calorie diet; oxidative stress; lipids.