DETERMINATION OF THE LEVEL OF microRNA-21 IN THE PLASMA OF PATIENTS WITH OSTEOSARCOMA AND CHONDROSARCOMA BONE

Issue: 
12
Year: 
2017

A.N. Shirshova
Research Scientist, Laboratory of Pharmacogenomics,
Institute of Chemical Biology and Fundamental Medicine Siberian branch of the Russian Аcademy of Sciences (Novosibirsk)
E-mail: arina.shirshova@gmail.com
D.V. Shamovskaya
Research Scientist, Laboratory of Pharmacogenomics,
Institute of Chemical Biology and Fundamental Medicine Siberian branch of the Russian Аcademy of Sciences (Novosibirsk)
M.L. Filipenko
Ph.D. (Biol.), Head of Laboratory of Pharmacogenomics,
Institute of Chemical Biology and Fundamental Medicine Siberian branch of the Russian Аcademy of Sciences (Novosibirsk)
A.A. Alferov
Post-graduate Student, Clinical Biochemistry Laboratory, N.N. Blokhin National Medical Cancer Research Center (Moscow)
A.V. Bondarev
Oncologist, Clinical Biochemistry Laboratory, N.N. Blokhin National Medical Cancer Research Center (Moscow)
I.V. Boulitcheva
Dr.Sc. (Med.), Pathologist, Department of Pathological Anatomy of Human Tumors,
N.N. Blokhin National Medical Cancer Research Center (Moscow)
Yu.N. Soloviev
Dr.Sc. (Med.), Professor, Academician of RAS, Department of Pathological Anatomy of Human Tumors,
N.N. Blokhin National Medical Cancer Research Center (Moscow)
N.E. Kushlinskii
Dr.Sc. (Med.), Professor, Corresponding Member of RAS, Head of Clinical Biochemistry Laboratory,
N.N. Blokhin National Medical Cancer Research Center (Moscow)

Osteosarcomas and chondrosarcomas are common malignant tumors of bones with different etiologies, therapy schemes and prognosis. The prognosis for osteosarcomas and chondrosarcomas, as in any malignant process, largely depends on the stage of the disease at the stage of initiation of therapy. Timely diagnosis remains the decisive factor in the fight against oncoclogical deseases. MicroRNA is a class of regulatory RNAs that are promising as molecular markers of various human diseases, including oncology. The article presents the results of a comparative quantitative assessment of microRNA-21 levels in the blood plasma of patients with osteosarcoma, chondrosarcoma and a control group that included healthy donors. It was shown that the level of microRNA-21 was statistically significantly 2.98 times higher in plasma samples of patients with osteosarcoma and 3.44 times in plasma samples of patients with chondrosarcoma of bone compared to the control group. ROC analysis was performed to assess the diagnostic potential of determining the level of microRNA-21 for osteosarcoma. The AUC value was 0.89.

Keywords: 
Key words: osteosarcoma
chondrosarcoma
bones
microRNA-21
miR-21
cel-238
polymerase chain reaction
reverse transcription.