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INVESTIGATION OF RELEASE KINETICS OF DOXORUBICIN FROM DRUG CARRIER BASED ON Fe(0) MICROPARTICLES UNDER INFLUENCE OF ULTRASOUND IRRADIATION AND DIFFERENT VALUES OF pH

DOI: https://doi.org/10.29296/25877313-2019-05-03
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Issue: 
5
Year: 
2019

S.S. Vlasov Post-graduate Student, Research School of Chemistry and Applied Biomedical Sciences of Tomsk Polytechnic University; Production Engineer, Technology Implementation Centre, Central Scientific Research Laboratory, Siberian State Medical University (Tomsk) E-mail: unreal800@gmail.com A. Di Martino Ph.D., Research Scientist, Research School of Chemistry and Applied Biomedical Sciences of Tomsk Polytechnic University; Junior Research Scientist, Centre of Polymer Systems, Tomas Bata University (Zlin, Czech Republic) M.S. Yusubov Dr.Sc. (Chem.), Professor, Headmaster of Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University; Chief Research Scientist, Central Scientific Research Laboratory, Siberian State Medical University (Tomsk) A.M. Guryev Dr.Sc. (Pharm.), Head of Technology Implementation Centre, Central Scientific Research Laboratory, Siberian State Medical University (Tomsk) S.V. Krivoschekov Junior Research Scientist, Central Scientific Research Laboratory, Siberian State Medical University (Tomsk) E.V. Sviridova Post-graduate Student, Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University P.S. Postnikov Ph.D. (Chem.), Associate Professor, Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University M.V. Belousov Dr. Sc. (Pharm), Head of Department of Pharmaceutical Analysis, Siberian State Medical University (Tomsk)

Aim. To obtain new drug carrier for doxorubicin based on modified Fe(0) microparticles and evaluate its release kinetics under influence of differ-ent values of pH and ultrasound irradiation. Material and methods. Size and zeta-potential of microparticles were determined on Zetasizer Nano ZS. Surface modification (covalent binding of residues of benzoic acid) was confirmed by FTIR spectroscopy. Encapsulation efficiency (EE) and loading capaci-ty (LC) of doxorubicin (DOX) was determined by UV spectroscopy (480 nm). Release studies were carried out in Stuart SI 500 incubator at a constant temperature (37 °C), stirring rate (100 rpm) and different pH values (3.3; 5.5; 7.4). For investigation of influence of ultrasound (US) irradiation on the re-lease kinetics ultrasound field with frequency and power 75 kHz and 2 W/cm2 respectively was used. Ultrasonic bath Elmasonic S10H was used as a source of ultrasound irradiation. Results and discussions. Size and zeta-potential of Fe-CS-DOX conjugate were 4.43 and -9.07 respectively. Loading capacity of doxorubicin was 0.54 mg/mg. Percentage of released drug with and without US irradiation were 96 and 18% respectively (in 12 hours after starting of the experiment). Conclusion. In this study, the release of doxorubicin from drug carrier, based on Fe (0) microparticles at different pH values was investigated and the influence of ultrasound irradiation on the release kinetics was confirmed. In 12 hours after starting of the release, the amount of released drug was increased more than 4 times. So, the obtained conjugate Fe-CS-DOX leaves great promise for its further use as a drug carrier.
Keywords: 
doxorubicin
chitosan
iron zero-valent microparticles

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