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UV-B RAYS ACTION ON FLAX CULTURE IN VITRO AND THE ACCUMULATION OF PHENOLIC COMPOUNDS IN THEM

DOI: https://doi.org/10.29296/25877313-2019-10-06
Issue: 
10
Year: 
2019

E.A. Goncharuk Ph.D. (Biol.), Senior Research Scientist, К.А. Timiryazev Institute of Plant Physiology Russian Academy of Sciences (IPP RAS, Moscow) E-mail: goncharuk.ewgenia@yandex.ru L.V. Nazarenko Ph.D. (Biol.), Associate Professor, Moscow City University, Institute of Natural Sciences and Sports Technologies (MGPU, Moscow) E-mail: nlv.mgpu@mail.ru N.V. Zagoskina Dr.Sc. (Biol.), Professor, К.А. Timiryazev Institute of Plant Physiology Russian Academy of Sciences (IPP RAS, Moscow) E-mail: biophenol@gmail.com

The flax possesses the ability to biosynthesis of secondary metabolites, including phenolic compounds, which have high biological activity. Methods of biotechnology allow us to research the influence of various stressors on plants. The aim of the work was to study the effect of UV-B rays on the morphophysiological characteristics of the contrast resistance to stress strains of callus cultures of flax (Linum usitatissimum L.) and the accu-mulation of phenolic compounds in them. A feature of callus was evaluated by morphological characteristics and water content. Phenolic compounds were extracted with 96% ethanol and their total content was determined spectrophotometrically. It was found that in response to the action of UV-B radiation in flax callus cultures oc-curs activation of adaptation processes. The studied parameters change to a greater measure in flax culture with reduced stress resistance. Thus, the influence of UV-B rays on plant cell cultures leads to an increase in the accumulation of phenolic compounds in them.
Keywords: 
flax
Linum usitatissimum L.
callus culture
UV-B rays
phenolic compounds
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