COMPARATIVE STUDY OF THE COMPOSITION OF ESSENTIAL OIL PHLOJODICARPUS SIBIRICUS (STEPH. EX SPRENG.) KOSO-POL. FROM THE EASTERN BAIKAL REGION AND ITS ANTI-RADICAL ACTIVITY

DOI: https://doi.org/10.29296/25877313-2019-12-06
Issue: 
12
Year: 
2019

V.V. Taraskin Ph.D. (Pharm.), Senior Research Scientist, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management of the Siberian branch of the RAS; Senior Lecturer, Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude) E-mail: vvtaraskin@mail.ru A.V. Polоnova Post-graduate Student, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management Siberian branch of the RAS (Ulan-Ude) S.M. Gulyaev Ph.D. (Med.), Senior Research Scientist, Laboratory of Experimental Pharmacology, Institute of General and experimental biology of the Siberian branch of the RAS (Ulan-Ude) E.P. Dylenova Post-graduate Student, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management Siberian branch of the RAS (Ulan-Ude); Lecturer, Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude) Yu.N. Nikolaev Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude) Zh.A. Tykheev Post-graduate Student, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management Siberian branch of the RAS; Assistant, Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude) B.M. Urbagarova Pos-tgraduate Student, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management Siberian branch of the RAS; Lecturer, Department of Pharmacy, Banzarov Buryat State University (Ulan-Ude)

Aim of the research: a comparative study of the composition of essential oil obtained from the herb, rhizomes and roots of Phlojodicarpus sibiricus (Steph. ex Spreng.) Koso-Pol. from the population of Pribaikalsky region and determination of its antiradical activity. Material and methods. The objects of studying were herb, rhizomes and roots of Phlojodicarpus sibiricus (Steph. ex Spreng.) Koso-Pol. growing within the Pribaikalsky region of the Republic of Buryatia (Lake Baikal’s coast), collected during bearing stage of growth (July, 2017). Essential oils from the herb, rhizomes and roots were obtained using commonly used method - steam distillation. The content of essential oil was determined using volumet-ric method. The qualitative composition and relative quantitative content of the components of essential oils was identified by gas-chromato-mass spectrometric method. The analysis of antiradical activity of obtained essential oils was conducted using DPPH-test. Results. For the first time the chemical composition of essential oils obtained from herb, rhizomes and roots of Phlojodicarpus sibiricus from the population of Pribaikalsky region was studied. According to the results the stability of the composition of essential oils depending on the place of growth was shown. γ-Terpinene (14.8%), cis-Nerolidol (9.84%), Germacrene D (9.70%) and p-Cymol (7.04%) were the main components of essential oil from the herb. In the essential oils from rhizomes and roots the main constituents were γ-Terpinene (29.60%), Terpinolene (22.85%), p-Cymol (15.95%) and Limonene (7.64%). High antiradical activity of the investigated essential oils was established. IC50 for essential oils from the aerial part of Phlojodicarpus sibiricus was 13.44 μg/ml, and from rhizomes and roots - 16.07 μg/ml. In addition, high antiradical activity of essential oils contained in pharmacologically active water-alcohol ex-tracts may partly explain their neuroprotective activity. Conclusion. The chemical composition of the essential oils obtained from herb, rhizomes and roots of Phlojodicarpus sibiricus from the Pribaikalsky population was studied. High antiradical activity of analyzed essential oils was estimated. As a re-sult, we found it rationally to obtain essential oils from the raw material during the development of coumarin-containing remedies.

Keywords: 
Phlojodicarpus sibiricus (Steph. ex Spreng.) Koso-Pol.
essential oils
anti-radical activity

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