THE INFLUENCE OF PUMPKIN POLYSACCHARIDES ON OXIDATIVE STRESS INDICES IN ACUTE HYPOXIA WITH HYPERCAPNIA IN RATS

The aim. In the model of acute hypoxia with hypercapnia in an experiment on male rats, the protective effect of polysaccharides of pumpkin fruits in relation to the oxidative modification of protein molecules of skeletal muscle tissue is shown. Material and Methods. The experiment examined tissues of the cardiac and skeletal muscles taken from 18 sexually mature male rats of Wistar weighing 200-220 g, which were kept in the vivarium of Ryazan State Medical University. The animals were divided into three groups of 6 individuals. The first group of animals received a 10% solution of polysaccharides extracted from pumpkin fruits by intragastric feeding through a tube daily for 30 days at the dose of 0.1 g / kg body weight. The polysaccharides were extracted according to the following scheme: to remove extractives and colored molecules from the raw material, it was preliminarily treated with 40-60% ethanol solutions. Then, a weighed portion of air-dried fruits was placed in a round bottom flask. After that it was poured with a 1% solution of ammonium oxalate and extracted in a boiling water bath for one and a half hours. Af-ter that the mixture was filtered, the filtrate was evaporated, and the obtained extract was precipitated with one and a half volume of 96% ethanol. The precipitate (PS) was separated, purified with ethanol, ether and acetone. The isolated PS was dried in vacuo for 12 hours over P2O5. On the last day of PS administration, the animals were exposed to acute normobaric hypoxia with hypercapnia once. For this, each animal was placed in a sealed cham-ber with a volume of 1000 ml for 30 minutes. The second group of animals received saline according to the introduction of PS. On the last day of the experiment, the animals were placed in a chamber with natural ventilation for 30 minutes. The third group of the animals remained intact until the end of the experiment and underwent hypoxia in the same way as the first group of the animals. After that tissues were taken from the anesthetized rats. Homogenates were obtained from skeletal and cardiac muscle tissues using a homogenizer, which were centrifuged for 10 minutes at 1000 g to pre-cipitate nuclei and intact cells. The resulting supernatant was used for further studies. To assess the antioxidant ability of PS, the activity of the en-zyme of the antioxidant system of cells, superoxide dismutase (SOD), was determined; to evaluate the protective effect of PS in acute hypoxia with hypercapnia, oxidative modification of proteins (OMB) was determined as markers of oxidative stress. For calculations in each sample, the concentra-tion of total protein was determined by the Lowry method. Statistical data processing was performed using Microsoft Office Excel 2010. The level of differences was considered statistically significant with an error probability of p