HEPATOPROTECTIVE EFFECT OF THE EXTRACT OF AGRIMONY ORDINARY TETRACHLOROMETHANE IN EXPERIMENTAL HEPATITIS

DOI: https://doi.org/10.29296/25877313-2020-02-06
Issue: 
2
Year: 
2020

E.N. Kurmanova Research Scientist, Department of Experimental and Clinical Pharmacology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E-mail: kurmanova1968@yandex.ru L.B. Strelkova Ph.D. (Med.), Leading Research Scientist, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) E.V. Ferubko Ph.D. (Med.), Head of the Department of Experimental and Clinical Pharmacology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow) O.P. Sheichenko Ph.D. (Chem.), Leading Research Scientist, Department of Phytochemistry and Standardization, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Moscow)

Relevance. The search for sources of new effective biologically active substances from plant raw materials for the creation of drugs of rehabilitation and rehabilitation orientation for the treatment of disorders in the liver is currently relevant. A promising object is the common repeche (Agrimónia eu-patória L.), which is used in folk medicine for the treatment of liver and gall bladder diseases. In GNU VILAR developed repeshko ordinary herb dry ex-tract. The aim of the study was to study the effect of repeshka extract on the liver condition of rats under experimental toxic hepatitis. Material and methods. The pharmacological properties of the extract were studied at doses of 50 mg/kg and 100 mg/kg, the comparison drug was silimar at a dose of 100 mg/kg. The drugs were administered to animals for 3 days. Experimental hepatitis was caused by a single subcutaneous in-jection of carbon tetrachloride to rats one hour after the last injection of the studied substances. After 48 hours, the rats were euthanized in a CO2 chamber and the liver was extracted, from which microsomal fractions were isolated, the content of microsomal protein and cytochrome P450 was de-termined, as well as the rate of monooxygenase reactions catalyzed by cytochrome P450, reflecting the activity of the detoxifying microsomal system of the liver. Taking into account all the studied parameters, the hepatoprotective and detoxifying properties of the extract and the comparison drug were evaluated. Results. In the experiment it was found that in the treatment of hepatitis agrimony extract in doses of 50 mg/kg and 100 mg/kg normalizes the con-tent of microsomal protein and cytochrome P450 content in liver microsomes increased by 28% and 2 times, respectively, compared to control. The introduction of silymar also normalizes the content of microsomal protein, and the content of cytochrome P450 increases by 16%. It was found that when treating hepatitis with repeshka extract at a dose of 50 mg/kg and 100 mg/kg, the anilization rate increases by 12% and 30%, and the demeth-ylation rate increases by 40% and 58%, respectively. This indicates enzymatic activity centres demethylation and cytochrome P450 inducing effects of biologically active substances contained in the extract of agrimony on monooxygenase system of the liver. Silimar also showed high hydroxylase and demethylase activity. Conclusions. Thus, the dry extract of the grass repeshka ordinary in conditions of Tetrachloromethane hepatitis in rats has hepatoprotective and de-toxifying properties.

Keywords: 
agrimony ordinary herb extract dry
experimental hepatitis
hepatoprotective effect
anti-toxic effect

References: 
  1. Yudin M.A., Bykov V.N., Sazonova A.V. Podhody k analizu mekhanizmov formirovaniya otdalennyh posledstvij intoksikacii unichtozhaemymi organofosfatami. Voprosy obespecheniya himicheskoj bezopasnosti v Rossijskoj Federacii. 2007. S. 187–189.
  2. Rejzis A.R., Borzakova S.N., Aksyonova V.A. Lekarstvenno-inducirovannye porazheniya pecheni – aktual'naya problema sovremennoj mediciny. Gastroenterologiya. 2010; 3:4954.
  3. Dikorastushchie poleznye rasteniya Rossii / Pod red. A.L. Budancev, E.E. Lesiovskaya. SPb: Izd-vo SPHFA. 2001. 663 s.
  4. Polyanskih L.S., Petrosyan M.A., Zhestkova N.V., Balashova N.N. Eksperimental'nye modeli patologii pecheni. Eksperimental'naya i klinicheskaya farmakologiya. 2017; 2:41.
  5. Karuzina I.I., Bachmanova G.I., Mengazetdinov D.E., Myasoedova K.N., Zhihareva V.O., Kuznecova G.I., Archakov A.I. Vydelenie i svojstva citohroma R450 iz mikrosom pecheni krolikov. Biohimiya. 1979; 6:10491057.
  6. Avdeev V.G. Metody opredeleniya koncentracii belka. Voprosy medicinskoj himii. 1977; 4:562571.
  7. Omura T., Sato R. The carbon monoxide-binding pigment. Journal Biological Chemistry.1964; 7:23702378.