DEVELOPMENT OF A SCHEME FOR ACCELERATED IDENTIFICATION OF XANTHOMONAS CAMPESTRIS BACTERIA USING BACTERIOPHAGE IN LABORATORY CONDITIONS

DOI: https://doi.org/10.29296/25877313-2020-03-03
Issue: 
3
Year: 
2020

P.S. Maiorov applicant, Ulyanovsk State Agricultural University (Ulyanovsk) E-mail: pavelmayorovv@yandex.ru N.A. Feoktistova Ph.D. (Biol.), Associate Professor, Ulyanovsk State Agricultural University (Ulyanovsk) E-mail: feokna@yandex.ru D.A. Vasilyev Dr.Sc. (Biol.), Professor, Ulyanovsk State Agricultural University (Ulyanovsk) E-mail: dav_ul@mail.ru

The aim of the study was to develop a phage titer rise reaction (PRR) scheme for the indication of Xanthomonas campestris pv. campestris bacteria. Material and methods. The PRR scheme was developed using the bacteriophage Cl34-Ulgau and the indicator culture of X. campestris pv. campestris bacteria Xc2 is based on the methods applied by the staff of the Department of Microbiology of the Ulyanovsk State Agricultural University. Results. The paper presents the developed scheme of PRR. The optimum parameters for the formulation of the PRR. The working dilution of the phage biopreparation Kl34–ULSAU was 104 BOE/ml. The dilution of the indicator culture of bacteria 107 mc/ml. the cultivation time of crops with indicator cul-ture and phage biopreparation was 18 hours. Conclusion. Identification of the bacteria X. campestris pv. campestris based on the developed phage titer rise reaction scheme is 43 hours.

Keywords: 
bacteriophages
Xanthomonas campestris pv. campestris
phage titer increase reaction
accelerated identification
biopreparation

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