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N.A. Beketova Ph.D. (Chem.) Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow) O.A. Vrzhesinskaya Ph.D. (Biol.), Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow) V.M. Kodentsova Dr.Sc. (Biol.), Professor, E-mail: O.V. Kosheleva Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow) L.V. Shevyakova Ph.D. (Biol.), Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow) S.N. Leonenko Post-graduate Student, Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow) S.J. Soto Ph.D. (Med.), Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow) A.A. Sokolnikov Ph.D. (Biol.), Federal Research Centre of Nutrition, Biotechnology and Food Safety (Moscow)

Insufficient intake of vitamins of group B and vitamin D is typical for the population of Russia. Data on disturb of mineral status and redistribution of trace elements in rat organs and tissues under deficiency of B vitamins had been appeared. The aim of the study was to characterize the effect of multiple vitamin deficiency in growing rats on the mineral status and some blood parameters. Material and methods. Multiple vitamin deficiency in Wistar male rats (n = 12) with an initial body weight (51.4 ± 0.5) was caused by a 5-fold de-crease in the vitamin content in the vitamin mixture of a semi-synthetic diet for 23 days. Animals of the control group (n = 12) received a full semi-synthetic diet. The concentration of vitamins A in blood plasma and lyophilized liver and the whole brain of rats was de-termined by HPLC, vitamins B1 and B2 in the liver, brain, urine and 4-pyridoxic acid in urine fluorimetric method. The content of minerals in the liver and brain was determined by the atomic absorption method. Results. Multiple vitamin deficiency was accompanied by a decrease copper by 6.8 times and magnesium by 26.9%, in the brain, and increase of 11.6% in calcium, an increase of the blood plasma concentration of calcium, protein and phosphorus in the by 2.96.9%, creatinine  by 20.3%, a 2.2-fold increase of the ratio of γ- and α-tocopherols (due to an increase of 1.5 times the concentration of γ-tocopherol), an increase in the De Ritis ratio by 32.9%. Conclusions. Multiple vitamin deficiency affects the redistribution of minerals in organs, which justifies the need to maintain the vitamin status at the optimal level and confirms the benefits of using vitamin-mineral complexes containing not only a complete set of vitamins, but also deficient minerals and trace elements.

multiple vitamin deficiency
vitamin-mineral status
blood plasma

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