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STUDYING OF THE ANTI-ISCHEMIC EFFECT OF OROSTACHYS SPINOSA DRY EXTRACT IN CAROTID ARTERIES BILATERAL OCCLUSION IN WHITE RATS

DOI: https://doi.org/10.29296/25877313-2022-07-01
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Issue: 
7
Year: 
2022

Ya.G. Razuvaeva
Dr.Sc. (Biol.), Senior Research Scientist,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Russia)
E-mail: tatur75@mail.ru
E.A. Bayandueva
Post-graduate Student,
Banzarov Buryat State University (Ulan-Ude, Russia)
A.A. Toropova
Ph.D. (Biol.), Senior Research Scientist,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Russia)
I.G. Nikolaeva
Dr.Sc. (Pharm.), Senior Research Scientist,
Institute of General and Experimental Biology SB RAS (Ulan-Ude, Russia)

The aim of the study to evaluate the anti-ischemic effect of Orostachys spinosa dry extract in common carotid arteries bilateral occlusion in albino rats. Material and methods. The studies were carried out on 67 Wistar rats. O. spinosa extract was used as research object at doses 50, 100, 200 mg/kg. Introductory extract solution was administered intragastrically for 14 days prior to carotid arteries bilateral occlusion. The anti-ischemic effect of the O. spinosa extract was evaluated total mortality, dynamics of survival, mean life time, as well as the neurological status of animals using the modified McGraw scale and the degree of rats brain hydration. Results. It was found that the lowest animals mortality rate was observed in the experimental groups treated with O. spinosa extract at doses 100 and 200 mg/kg in carotid arteries common bilateral occlusion. The most pronounced statistically significant increase in life expectancy was observed in animals treated with O. spinosa extract at doses 50 and 100 mg/kg (by 38%, p≤0.05) arelative to that of control animals. The least pronounced neu-rological deficit degree was observed in animals treated with O. spinosa extract at doses 50 and 100 mg/kg. The most pronounced statistically signifi-cant decrease in the brain hydration level was observed in animals receiving O. spinosa extract at a dose 100 mg/kg. Life expectancy in animals, treated with dry O. spinosa extract at doses 50 and 100 mg/kg was incresed by 38% (p≤0.05) that of animals in the control group. The neurological deficit least pronounced degree was observed in ani-mals treated with O. spinosa extract at doses of 50 and 100 mg/kg. The most pronounced statistically significant decrease in the level of brain hydra-tion was observed in animals treated with dry O. spinosa extract at a dose 100 mg/kg. Conclusion. O. spinosa extract has a neuroprotective effect in cerebral ischemia, reducing the animals death percentage, increasing animals life ex-pectancy, and reducing the neurological deficit degree and the cerebral edema severity.
Keywords: 
Orostachys spinosa (L.) Sweet
anti-ischemic effect
ischemia
brain
rats

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References: 
  1. Beljaeva I.A., Martynov M.Ju., Pehova Ja.G., Vershinin A.A., Rachin A.P., Eremushkin M.A., Gusev E.I. Svjaz' dvigatel'nogo stereotipa i lokalizacii ochaga
  2. v rannem vosstanovitel'nom periode legkogo ishemicheskogo insul'ta. Zhurnal nevrologii i psihiatrii im. C.C. Korsakova. 2019; 119(32): 53–61.
  3. doi: 10.17116/jnevro201911903253.
  4. Barnaulov O.D., Barnaulova O.S. Fitoterapija ishemicheskoj bolezni serdca i mozga. Tradicionnaja medicina. 2020; 60(1): 12–24.
  5. Sambukova T.V., Ovchinnikov B.V., Ganapol'skij V.P., Jatmanov A.N., Shabanov P.D. Perspektivy ispol'zovanija fitopreparatov v sovremennoj farmakologii. Obzory po klinicheskoj farmakologii i lekarstvennoj terapii. 2017; 15(2): 56–63. doi: 10.17816/RCF15256-63.
  6. Nikolaeva I.G., Cybiktarova L.P., Nikolaeva G.G., Manzhigeev P.G. Fitohimicheskoe issledovanie nadzemnoj chasti gornokolosnika koljuchego. Voprosy obespechenija kachestva lekarstvennyh sredstv. 2018; 22(4): 52–56.
  7. Nikolaeva I.G., Tsybiktarova L.P., Taraskin V.V., Radnaeva L.D., Tykheev Zh.A., Nikolaeva G.G. Manzhigeev P.G. Lipids from Orostachys spinosa. Chemistry of Natural Compounds. 2018; 54(5): 961–963. doi: 10.1007/s10600-018-2522-9.
  8. Budancev A.L., Lesiovskaja E.E. Dikorastushhie poleznye rastenija Rossii. SPb: SPFHA, 2001: 663.
  9. Shreter A.I. Lekarstvennaja flora Sovetskogo Dal'nego Vostoka. M.: Medicina, 1975: 328.
  10. Leventa A.I., Odinec A.D., Ohremchuk L.V., Usov L.A. Vlijanie izvlecheniij iz gornokolosnika i rododendrona Adamsa na povedencheskie reakcii laboratornyh zhivotnyh. Sibirskij medicinskij zhurnal (Irkutsk). 2010; 95(4): 103–105.
  11. Odinec A.D., Leventa A.I., Shhukin D.A., Shabaturova O.V. K antigipoksichekomu dejstviju preparatov iz rastitel'nogo syr'ja Bajkal'skoj Sibiri. Sibirskij medicinskij zhurnal (Irkutsk). 2011; 104(5): 112–115.
  12. Odinec A.D., Usov L.A., Izatulin A.V., Leventa A.I., Shabaturova O.V. Vlijanie preparatov iz gornokolosnika koljuchego i rododendrona Adamsa na techenie stress-reakcii jeksperimental'nyh zhivotnyh. Bjulleten' VSNC SO RAMN. 2010; 76(6): 175–181.
  13. Usov L.A., Leventa A.I., Odinec A.D. Anksioliticheskie i mnemotropnye jeffekty izvlechenij iz gornokolosnika i rododendrona Adamsa v jeksperimente na labora-tornyh zhivotnyh. Sibirskij medicinskij zhurnal (Irkutsk). 2010; 96(5): 125–128.
  14. Rukovodstvo po provedeniju doklinicheskih issledovanij lekarstvennyh sredstv. Chast' pervaja. Pod red. A.N. Mironova. M.: Grif i K, 2012: 944.
  15. Gannushkina I.V. Mozgovoe krovoobrashhenie pri raznyh vidah cirkuljatornoj gipoksii mozga. Vestnik Rossijskoj Akademii medicinskih nauk. 2000; 9: 22–27.
  16. Luo Y., Cui H-X., Jia A., Jia S.-S., Yuan K. The protective effect of the total flavonoids of Abelmoschus esculentus L. flowers on transient cerebral ischemia-reperfusion injury is due to activation of the Nrf2-ARE pathway. Oxidative medicine and cellular longevity. 2018; doi: 10.1155/2018/8987173.
  17. Manish P., Lim W.L., Teoh S.L., Yahaya M.F. Flavonoids and its neuroprotective effects on brain ischemia and neurodegenerative diseases. current drug targets. 2018; 19(14): 1710–1720. doi: 10.2174/1389450119666180326125252.
  18. Pan L., Cho K.-S., Yi I., To C.-H., Chen D.F., Do C.-W. Bai-calein, baicalin, and wogonin: protective effects against is-chemia-induced neurodegeneration in the brain and retina. Oxidative medicine and cellular longevity. 2021. doi: 10.1155/2021/8377362.
  19. Wu C.-T., Chen M-C., Liu S.-H., Yang T.-H., Long L.-H., Guan S.-S, Chen C.-M. Bioactive flavonoids icaritin and icariin protect against cerebral ischemia-reperfusion-associated apoptosis and extracellular matrix accumulation in an ischemic stroke mouse model. Biomedicines. 2021; 9(11): 1719.
  20. doi: 10.3390/biomedicines9111719.
  21. Asgharzade S., Khorrami M.B., Forouzanfar F. Neuroprotective effect of herniarin following transient focal cerebral ischemia in rats. Metabolic Brain Disease. 2021; 36: 2505–2510. doi: 10.1007/s11011-021-00841-1.
  22. Barchestani Z.N., Rafieirad M. The Effect of herniarin on spatial working memory, pain threshold, and oxidative stress in ischemic hypoperfusion model in rats. Caspian Journal of Neurological Sciences. 2021; 7 (1): 42–50. doi: 10.32598/CJNS.7.24.5.
  23. Liang S., Chen Z., Li H., Cang Z., Yin K., Wu M., Luo S. Neuroprotective effect of umbelliferone against cerebral ischemia/reperfusion induced neurological deficits: in-vivo and in-silico. Journal of biomolecular structure and dynamics. 2021; 39 (132020): 4715–4725. doi: 10.1080/07391102.2020.1780153