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METABOLIC PROFILING OF ARTEMISIA SANTHONICUM AND ARTEMISIA LERCHIANA BY GC/MS

DOI: https://doi.org/10.29296/25877313-2019-07-03
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Issue: 
7
Year: 
2019

M.T. Nikolova Ph.D., Associate Professor, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria E-mail: mtihomirova@gmail.com I.Y. Aneva Ph.D., Chief Assistant Professor, nstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria E-mail: ina.aneva@abv.bg M.N. Dimitrova Chemist, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria E-mail: marina17dimitrova@gmail.com S.H. Berkov Ph.D., Professor, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria E-mail: berkov_str@yahoo.com

In the flora of Bulgaria, the genus Artemisia L. is represented by 13 species. This study is devoted to the study of two morphologically similar species - Artemisia lerchiana Stechm and Artemisia santonicum L. Materials and methods. The objects of study were methanol and acetone extracts from the dried aerial part and the fractions obtained from them. We used TLC and GC-MS methods. The antioxidant activity of the extracts was assessed by the method with DPPH reagent. Results and discussion. In the lipophilic fractions of the methanol extract of A. lerchiana and A. santonicum fatty acids, higher alcohols and sterols was found, and A. santonicum is distinguished by a high content of C18:3, C18:1, C18:2 fatty acids. Organic acids, phenolic acids, mono- and disaharas were found in the polar fractions. In the acetone extract triterpenes, organic and phenolic acids, and sugars were determined. Using TLC profile differences for the studied species were established. When comparing the antioxidant properties, it was found that the methanol extracts of the studied species have the greatest activity compared to the acetone extracts, while the extracts from A. santhonicum exhibited stronger activity than A. lerchaina. Findings. A comparative study of the profile of the metabolites of A. santhonicum and A. lerchaina was carried out. It has been shown that the methanol extract of A. santhonicum has a richer metabolite composition and a higher antioxidant activity. The data obtained are new to the studied representatives of the genus Artemisia L.
Keywords: 
exudate flavonoids
fatty acids
phenolic acids
DPPH

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