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EFFECT OF THE DRY RHAPONTICUM UNIFLORUM (L.) DC EXTRACT ON THE STATE OF THE IMMUNE AND ANTIOXIDANT SYSTEMS OF THE BODY IN EXPERIMENTAL IMMUNODEFICIENCY

DOI: https://doi.org/10.29296/25877313-2022-01-07
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Issue: 
1
Year: 
2022

V.B. Khobrakova Dr.Sc. (Biol.), Associate Professor, Head of the Laboratory of Experimental Pharmacology, Institute of General and Experimental Biology SB RAS; Professor of the Department of Human General Pathology, Medical institute, Buryat State University named after Dorzhi Banzarov (Ulan-Ude) E-mail: val0808@mail.ru Yu.A. Tugarina Post-graduate Student, Laboratory of Experimental Pharmacology, Institute of General and Experimental Biology, SB RAS (Ulan-Ude, Russia) E-mail: ytugarina@mail.ru A.A. Toropova Ph.D. (Biol.), Senior Researcher, Laboratory of the Biologically Active Substances Safety, Institute of General and Experimental Biology SB RAS; Senior Lecturer of the Department of Infectious Diseases, Medical institute, Buryat State University named after Dorzhi Banzarov (Ulan-Ude, Russia) E-mail: anyuta-tor@mail.ru D.N. Olennikov D.Sc (Pharm.), Leading Researcher, Laboratory of Biomedical Research, Institute of General and Experimental Biology, SB RAS (Ulan-Ude, Russia) E-mail: olennikovdn@mail.ru L.R. Abidueva Ph.D. (Biol.), Associate Professor, Infectious Diseases Department, Medical institute, Dorji Banzarov Buryat State University (Ulan-Ude, Russia) E-mail: abidueva75@mail.ru

The purpose of the study was to evaluate the immunomodulatory effect and antioxidant activity of the dry extract from the herb of Rhaponticum uni-florum (L.) DC. Materials and methods. Experiments were carried out on F1 (СВАхС57Вl/6) mice and Wistar rats. Immune deficiency was simulated by a single in-traperitoneal introduction of cyclophosphamide in the dose 250 mg/kg. The first and second experimental groups administered the dry extract from R. uniflorum (100 mg/kg) and a comparative drug «Echinacea P» (200 mg/kg) respectively intragastrically once a day for 14 days against the background of cyclophosphamide immune suppression. The third and fourth intact groups received the R. uniflorum extract and «Echinacea P» respectively in the same doses for 14 days. The intact group administered the purified water according to the analogous scheme. The effect of the tested remedy on the cellular immunity was evaluated in the reaction of the delayed-type hypersensitivity (DTH), the humoral immunity was estimated by the number of an-tibody-forming cells (AFC). The antioxidant status of the body was assessed by the content of malondialdehyde, the activity of SOD, catalase, and GPx in the spleen homogenate. The antiradical activity of R. uniflorum extract was studied in vitro by its ability to reduce 2,2’-diphenyl-1-picrylhydrazyl (DPPH˙) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonate (ABTS•+), neutralize O2- and Fe2+. Results. The extract increased the DTH-index by 1.5 times and the value of absolute and relative numbers of AFC by 1.5 and 1.4 times as compared with the control group; it was comparable to the action of «Echinacea P» and did not change the immune indicators in intact mice. The extract reduced the concentration of malondialdehyde by 1.8 times, increased SOD and catalase activity by 1.6 times; GPx - 1.4 times; in vitro the extract exhibited antiradical effect. Conclusion. The R. uniflorum extract has an immunomodulatory effect on cellular and humoral immunity in cyclophosphamide immunosuppression. Its action is based on membrane-stabilizing and antioxidant activity.
Keywords: 
Rhaponticum uniflorum (L.) DC.
cyclophosphamide immunosuppression
immune modulating activity
antioxidant activity

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