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SHORT-CHAIN FATTY ACIDS QUANTITATIVE DETERMINATION METHODS IN ARCTIUM LAPPA L. LEAVES BIOMASS EXTRACTS

DOI: https://doi.org/10.29296/25877313-2022-11-03
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Issue: 
11
Year: 
2022

R.G. Khaibullin
Post-graduate Student, Department of Chemistry and Biotechnology,
Perm National Research Polytechnic University (Perm, Russian Federation)
E-mail: ruslan.khaibullin@yandex.ru
E.Y. Tumilovich
Ph.D. (Pharm.), Associate Professor, Department of Toxicological Chemistry,
Perm State Pharmaceutical Academy (Perm, Russian Federation)
E-mail: e.tumilovich@gmail.com
L.V. Volkova
Dr.Sc. (Med.), Professor, Department of Chemistry and Biotechnology,
Perm National Research Polytechnic University (Perm, Russian Federation)
E-mail: wolkowalw@mail.ru
T.L. Malkova
Dr.Sc. (Pharm.), Professor, Department of Toxicological Chemistry,
Perm State Pharmaceutical Academy (Perm, Russian Federation)
E-mail: malkova-tl@pfa.ru

Relevance. Fermentation of Arctium lappa L. (burdock) leaves with the Lactobacillus plantarum microorganisms makes it possible to obtain biologically active substances containing a range of compounds in its composition. These compounds determined by the used plant material composition and synthesized bac-terial metabolites, one of which are short-chain fatty acids (SCFA) that play important role in maintaining human health. In combination with the polysaccha-ride inulin, present in the leaves of A. lappa L, SCFA in the fermented product will have a complex effect, including prebiotic and metabiotic activity. This fermented biomass can be used as a pharmaceutical substance in a drugs production for the prevention and concomitant therapy of metabolic disorders. Aim of the research - Development of SCFAs determination methods in A. lappa L. leaf biomass samples aqueous extracts. Evaluation of the using microbi-al fermentation effectiveness to obtain biologically active substances. Material and methods. The objects of study - aqueous extracts (1:10) of A. lappa L. leaf biomass samples, obtained by drying, traditional fermentation, and microbial fermentation by L. plantarum 8P-A3 microorganisms. Research methods - gas-liquid and high-performance liquid chromatography Results. As a result, a method of some SCFA determination in plant extracts was developed. It has been established, that the acids-metabolites content in microbial-fermented A. lappa L. leaves biomass sample is higher than the acids content in samples obtained by drying and traditional fermentation. Conclusion. The developed conditions of acetic, butyric and lactic acids determination in plant extracts by gas-liquid and high-performance liquid chroma-tography can be used to analyze analogous products, as well as to develop determining the entire spectrum of SCFA methods. Plant raw materials fermenta-tion by L. plantarum microorganisms makes it possible to obtain SCFA-enriched substances, which can be used to produce therapeutic and prophylactic drugs.
Keywords: 
Arctium lappa L.
microbial fermentation
Lactobacillus plantarum
acids-metabolites
gas-liquid chromatography
high performance liquid chromatography with mass-selective detection.

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