A NEW METHOD OF TINCTURE PREPARATION ON THE BASE OF ARTEMISIAE JACUTICAE HERBA

The aim of the research was to develop a new method of tincture preparation from the closely related species of Artemisia absinthium L. – Ar-temisia jacutica Drob. The object of the study was Artemisiae jacuticae herba collected in Eravninsky region (Buryatia) in 2018 in a flowering stage. The quality of tincture was evaluated by the sum of extractives and the sum of flavonoids referred to cynaroside. Using commonly accepted method we determined the amount of the sum of extractives. And the quantitative content of the sum of flavonoids was determined using UV-spectrophotometry. Extraction on an orbital shaker significantly increases the duration and efficiency of extraction. During the analysis we found that the optimal ex-tractant is 50% ethanol that produces 32,14% of extractives and 0.53% of flavonoids. The best yield of extractives was found during extraction of 0.5 mm raw material, and was 30,29%, and the amount of flavonoids - 0.38%. According to the results, the optimal ration was 1:5 (33,25% of extractives and 0,59% - flavonoids). It was found that the optimal extraction time is 60 minutes, two times. More than that, we obtained tinctures using macera-tion method lasting from 1 to 7 days. It was revealed that the maximum yield of extractive substances was observed on the 7th day (29.19% - ex-tractives, 0.32% - flavonoids). Thus, we have set the optimal terms of tincture preparation from A. jacuticae herba for the better extractives’ efficiency, that included the type of extractant, raw materials’ fineness, the ratio of raw material: extractant and duration of extraction.